甘蓝型油菜'甘杂1号'抗旱性评价及转录组分析OA

[Objective]This study aimed to explore the drought resistance mechanism of the rapeseed variety'Ganza 1'and to mine drought-resistant genes.[Method]Drought stress treatment was employed;systematic evalua-tion of the drought tolerance of'Ganza 1'was conducted through phenotypic observations,measurements of chloro-phyll content,relative electrical conductivity,and stomatal aperture,combined with transcriptome analysis and quan-titative real-time PCR(qRT-PCR),to identify and validate drought-resistant genes.[Result]Compared with the con-trol group(normal watering),plants under drought stress exhibited significantly reduced plant height,decreased leaf area,lower chlorophyll content,increased relative electrical conductivity,and markedly smaller stomatal apertures(treatment group:inner stomatal length:(5.65±0.18)μm,inner stomatal width:(0.88±0.07)μm;control group:inner stomatal length:(4.66±0.14)μm,inner stomatal width:(1.28±0.01)μm).Further transcriptome(RNA-seq)analysis and screening for differentially expressed genes(DEGs)yielded a total of 2 004 DEGs.GO and KEGG functional annotations revealed that drought stress significantly affected pathways related to photosynthesis,pentose and glucuronate metabolism,plant hormone signal transduction,and flavonoid biosynthesis.qRT-PCR validation showed that the expression levels of eight drought-related genes(CNL46,WRKY8,NUDT21,CDC48,CIPK1,YA B B Y 2,M Y B 28,and ERF107)were consistent with the transcriptome results.[Conclusion]This study preliminari-ly identified functional genes associated with drought resistance in'Ganza 1',providing a reference for the mining of drought-resistant genes and advancing research on drought tolerance in rapeseed.