Comparison of gut viromes across captive mammals reveals extensive genetic diversity in bacteriophage dark matter and mammalian virusesOA
Comprehensive characterization of mammalian gut viromes is essential for early detection of commensal and potentially zoonotic viruses and for reducing the risk of cross-species transmission.Viral metagenomics was applied to profile gut viral communities from zoo mammals maintained across multiple zoological institutions in China.Viral communities differed markedly among host dietary guilds,with herbivores exhibiting the highest viral species diversity.In total,1027 viral sequences representing five major viral groups were recovered,including multiple mammal-associated astroviruses,picornaviruses,and parvoviruses with potential infectivity.Phylogenetic reconstruction based on viral hallmark genes demonstrated extensive genomic diversification across recovered lineages.Hosts for most microviruses were predicted to belong to the bacterial family Bacteroidaceae.In addition,10 previously unreported crAss-like phages were identified in mammalian samples and showed close evolutionary relationships with proposed crAssphages from the human gut virome.Antibiotic resistance genes identified in the mammalian gut viromes primarily belonged to tetracyclines.These findings substantially expand current understanding of viral community structure in captive animals in China and provide a foundation for proactive surveillance frameworks targeting emerging mammalian viruses with zoonotic potential.
Juan Lu;Hao-Ning Wang;Chun-Mei Wang;Juan Xu;Chukwudozie Kingsley Ikechukwu;Wang Li;Song-Yi Ning;Ping Wu;Yu-Wei Liu;Quan Shen;Li-Kai Ji;Xiao-Chun Wang;Shi-Xing Yang;Cheng-Ling Zhou;Xiao-Long Wang;Wen Zhang;Tong-Ling Shan
Department of Laboratory Medicine,School of Medicine,Jiangsu University,Zhenjiang,Jiangsu 212300,China Institute of Critical Care Medicine,Affiliated People’s Hospital,Jiangsu University,Zhenjiang,Jiangsu 212002,ChinaHeilongjiang Cold Region Wetland Ecology and Environment Research Key Laboratory,School of Geography and Tourism,Harbin University,Harbin,Heilongjiang 150086,China School of Geography and Tourism,Harbin University,Harbin,Heilongjiang 150086,ChinaShanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,ChinaClinical Laboratory Center,Affiliated Taizhou People’s Hospital of Nanjing Medical University,Taizhou,Jiangsu 225300,ChinaDepartment of Laboratory Medicine,School of Medicine,Jiangsu University,Zhenjiang,Jiangsu 212300,ChinaClinical Laboratory Center,Affiliated Taizhou People’s Hospital of Nanjing Medical University,Taizhou,Jiangsu 225300,ChinaDepartment of Laboratory Medicine,School of Medicine,Jiangsu University,Zhenjiang,Jiangsu 212300,ChinaDepartment of Laboratory Medicine,School of Medicine,Jiangsu University,Zhenjiang,Jiangsu 212300,ChinaDepartment of Laboratory Medicine,School of Medicine,Jiangsu University,Zhenjiang,Jiangsu 212300,ChinaDepartment of Laboratory Medicine,School of Medicine,Jiangsu University,Zhenjiang,Jiangsu 212300,ChinaDepartment of Laboratory Medicine,School of Medicine,Jiangsu University,Zhenjiang,Jiangsu 212300,ChinaDepartment of Laboratory Medicine,School of Medicine,Jiangsu University,Zhenjiang,Jiangsu 212300,ChinaDepartment of Laboratory Medicine,School of Medicine,Jiangsu University,Zhenjiang,Jiangsu 212300,ChinaClinical Laboratory Center,Affiliated Taizhou People’s Hospital of Nanjing Medical University,Taizhou,Jiangsu 225300,ChinaKey Laboratory of Wildlife Diseases and Biosecurity Management of Heilongjiang Province,Northeast Forestry University,Harbin,Heilongjiang 150006,ChinaDepartment of Laboratory Medicine,School of Medicine,Jiangsu University,Zhenjiang,Jiangsu 212300,China Institute of Critical Care Medicine,Affiliated People’s Hospital,Jiangsu University,Zhenjiang,Jiangsu 212002,ChinaShanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China
生物科学
Viral metagenomicsZoo animalsGut viromesBacteriophagesMammalian viruses
《Zoological Research》 2026 (2)
P.606-620,15
supported by the National Key Research and Development Programs of China(2023YFD1801301,2022YFC2603801)National Natural Science Foundation of China(82341106)。
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