Structural and functional insights into the distinct DNA recognition mechanisms of the terminase small subunit TerS from cyanophagesOA
Efficient genome packaging is a critical step in the phage life cycle,directly influencing the viral maturation and infectivity.In tailed phages,this process is driven by a packaging motor composed of a portal protein and a terminase complex.The terminase complex usually consists of a large subunit(TerL)and a small subunit(TerS),which cooperate to recognize,cleave,and translocate genomic DNA into the capsid.However,due to the remarkable diversity and complexity of phage packaging systems,the molecular mechanisms governing TerSmediated DNA recognition remain poorly understood.Here,we report the 3.51Åcryo-electron microscopy structure of the TerS from the short-tailed cyanophage Pam5,which infects the host Pseudanabaena mucicola Chao 1806.Pam5 TerS assembles into a nonameric ring with a radially symmetric spiral architecture.Biochemical assays show that Pam5 TerS recognizes the genomic DNA via a specific interaction between the N-terminal helix-turn-helix(HTH)domain of TerS and a 21-bp DNA sequence within the terS gene.In contrast,the TerS from another shorttailed cyanophage,Pam1,which infects the same host,binds to DNA in a sequence-independent manner.These findings reveal that cyanophages,even infecting the same host,could adopt two distinct DNA recognition strategies:HTH-mediated sequence-dependent or sequence-independent modes.This work provides structural and mechanistic insights into the diverse DNA-recognition strategies of TerS and advances our understanding of the evolutionary plasticity of viral genome packaging mechanisms.
De-Qin Dong;Feng Yang;Kang Du;Kang Xu;Wen-Bin Cheng;Yuxing Chen;Cong-Zhao Zhou;Yong-Liang Jiang
Department of Radiology,the First Affiliated Hospital of USTC,and School of Life Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230027,ChinaDepartment of Radiology,the First Affiliated Hospital of USTC,and School of Life Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230027,ChinaDepartment of Radiology,the First Affiliated Hospital of USTC,and School of Life Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230027,ChinaDepartment of Radiology,the First Affiliated Hospital of USTC,and School of Life Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230027,ChinaDepartment of Radiology,the First Affiliated Hospital of USTC,and School of Life Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230027,ChinaDepartment of Radiology,the First Affiliated Hospital of USTC,and School of Life Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230027,ChinaDepartment of Radiology,the First Affiliated Hospital of USTC,and School of Life Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230027,ChinaDepartment of Radiology,the First Affiliated Hospital of USTC,and School of Life Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230027,China
生物科学
cyanophageterminasegenome packaging motorcryo-electron microscopyDNA recognition
《Acta Biochimica et Biophysica Sinica》 2026 (6)
P.1281-1290,10
supported by the grants from the National Natural Science Foundation of China(Nos.32430001 and 92451302).
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