Exploring the soothing and reparative effects of Prinsepia utilis Royle oil based on network pharmacology and experimental validationOA

Background:Current interventions for sensitive skin hyperreactivity mainly target skin barrier restoration and inflammation relief.Natural plant extracts represented by Prinsepia utilis Royle oil contain abundant purified bioactive substances,whose functional mechanisms have become a hot research focus in cosmetic development.Objective:This research combined network pharmacology and in vitro/vivo experiments to verify the soothing and barrier-repairing cosmetic efficacy of Prinsepia utilis Royle oil,laying theoretical support for its application in sensitive skin care products.Materials and Methods:We screened anti-inflammatory and reparative active constituents of Prinsepia utilis Royle oil and their potential targets.Skin inflammation and repair-related targets were retrieved from GeneCards,and overlapping targets of active compounds and skin disease targets were visualized via Venn diagrams.PPI network,GO and KEGG enrichment analyses were conducted to interpret molecular interactions.Cell tests on P815 mast cells and zebrafish model assays were performed to detect NF-κB,histamine,TNF-α,OCLN levels and tail fin wound repair capacity for efficacy verification.Results:Palmitic acid,oleic acid,linoleic acid and vitamins are dominant components of Prinsepia utilis Royle oil.A total of 11 intersecting core targets were screened,among which AKT1,EGFR and TNF with high connectivity dominate skin inflammatory regulation and tissue repair.Enrichment analyses uncovered key functional terms and T cell receptor signaling pathways underlying its soothing activity.Cellular and zebrafish experiments proved that the oil remarkably suppresses pro-inflammatory mediators and accelerates wound recovery.Conclusions:Prinsepia utilis Royle oil exerts anti-inflammatory and repairing effects by regulating core targets including AKT1,EGFR and corresponding signaling pathways.Cellular and zebrafish experiments confirm its cosmetic soothing and barrier-protective effects,showing promising prospects for sensitive skin cosmetics.Further biological and clinical trials are still needed for deeper validation.