Dihydromyricetin:Extraction,efficacy tests and its application in cosmeticsOA

Background:Ampelopsis grossedentata(Hand.-Mazz.)W.T.Wang(AG)has a variety of ingredients and has immunomodulatory,hypoglycemic,and lipid-lowering effects.Dihydromyricetin(DMY)is one of the main functional ingredients in AG.Objective:To evaluate the efficacy of DMY from AG and develop a multi-functional and highly active product of DMY to use in cosmetics.Material and methods:The DMY samples were identified by analytical techniques including HPLC,IR,MS,and NMR.Efficacy tests include DPPH,ABTS,and ROS free radical scavenging;oil antioxidant test,promotion of caudal fin regeneration in zebrafish;promotion of Type I collagen in HFF-1 cells;LPS-induced inhibition of IL-6,inhibition of melanogenesis in B16 melanoma cells,inhibition of collagenase and tyrosinase,etc.Results:At 7.5μg/mL,the downregulation rates of IL-6 was 12.12%.At 10.0μg/mL,the DPPH and ABTS free radical scavenging rate were 63.66%and 100.00%,the ROS clearance rate of zebrafish was 12.00%,and the repairing rate was 23.20%in zebrafish.At 15.0μg/mL,the inhibition rate of collagenase and tyrosinase were 35.00%and 35.23%,and the promotion rates of type I collagen in HFF-1 cells was 19.09%.At 100.0μg/mL,the relative melanin content in B16 melanoma cells was 60.34%.Adding 0.01%of DMY in camellia seed oil and Olea europaea oil at 65°C for 28 days,the oxidation process was delayed by 88.35%and 65.69%,which were better than 0.05%BHT(72.30%and 62.16%).Conclusion:These findings suggest that DMY from AG has good anti-oxidation,anti-inflammatory,repairing,anti-wrinkling,and whitening effects,so it has broad application prospects as a natural functional ingredient in cosmetics.