BMPER induces the adipogenic differentiation of fibro/adipogenic progenitors and promotes intramuscular fat deposition in chickensOA
Background Fibro-adipogenic progenitors(FAPs)serve as the developmental origin of intramuscular adipocytes in skeletal muscle.Enhancing their adipogenic transition could increase intramuscular fat(IMF)deposition,thereby improving meat flavor in chickens.However,critical aspects of FAPs including their cellular composition,dynamic changes during skeletal muscle growth,and associated regulatory mechanisms remain poorly understood.Therefore,we comprehensively characterized FAPs in Langshan chickens from d 1 to d 98 using both in vivo and in vitro approaches combined with single-nucleus RNA sequencing(snRNA-seq)analysis.Results Our analysis of IMF contents and adipogenesis-related gene expression in the pectoralis major muscle of Langshan chickens revealed that the adipogenic properties of FAPs peaked at d 1,reached its lowest point at d14,and subsequently increased until d 98.The snRNA-seq analysis successfully identified the population of FAPs along with their 5 subtypes including the pre-adipogenic,adipogenic,and fibrotic FAPs.The ratio of the pre-adipogenic subtype decreased from d 14 to d 98,which was reversely correlated with the changes of the adipogenic subtype,suggesting a differentiating process.Furthermore,RNA velocity and pseudo-trajectory analysis revealed that the initial FAPs had superior fibrotic capacity but decreased over time which contrasted with their enhanced adipogenic capacity with development.Notably,BMPER was identified as an important regulator for the adipogenic differentiation of FAPs,which was also confirmed by in vitro over-expression studies.In addition,the expression of BMPER in the adipogenic portion of FAPs was found to be highly conserved across human and mouse skeletal muscles.Conclusions Our study provides the first comprehensive atlas of FAPs in the skeletal muscle of chickens and identifies BMPER as a key regulator for the adipogenic differentiation of FAPs.The findings will not only provide novel targets for breeding chickens with high IMF content but also offer significant insights into understanding the cell fate decision of FAPs under both physiological and pathological conditions across species.
Ying Liu;Jianliang Liu;Xuan Sun;Xiuze Zhang;Tong Xing;Lin Zhang;Feng Gao;Liang Zhao
College of Animal Science and Technology,State Key Laboratory of Meat Quality Control and Cultured Meat Development,Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province,Jiangsu Collaborative Innovation Center of Meat Production and Processing,Quality and Safety Control,Nanjing Agricultural University,No.1 Weigang,Nanjing 210095,P.R.ChinaCollege of Animal Science and Technology,State Key Laboratory of Meat Quality Control and Cultured Meat Development,Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province,Jiangsu Collaborative Innovation Center of Meat Production and Processing,Quality and Safety Control,Nanjing Agricultural University,No.1 Weigang,Nanjing 210095,P.R.ChinaCollege of Animal Science and Technology,State Key Laboratory of Meat Quality Control and Cultured Meat Development,Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province,Jiangsu Collaborative Innovation Center of Meat Production and Processing,Quality and Safety Control,Nanjing Agricultural University,No.1 Weigang,Nanjing 210095,P.R.ChinaCollege of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,P.R.ChinaCollege of Animal Science and Technology,State Key Laboratory of Meat Quality Control and Cultured Meat Development,Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province,Jiangsu Collaborative Innovation Center of Meat Production and Processing,Quality and Safety Control,Nanjing Agricultural University,No.1 Weigang,Nanjing 210095,P.R.ChinaCollege of Animal Science and Technology,State Key Laboratory of Meat Quality Control and Cultured Meat Development,Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province,Jiangsu Collaborative Innovation Center of Meat Production and Processing,Quality and Safety Control,Nanjing Agricultural University,No.1 Weigang,Nanjing 210095,P.R.ChinaCollege of Animal Science and Technology,State Key Laboratory of Meat Quality Control and Cultured Meat Development,Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province,Jiangsu Collaborative Innovation Center of Meat Production and Processing,Quality and Safety Control,Nanjing Agricultural University,No.1 Weigang,Nanjing 210095,P.R.ChinaCollege of Animal Science and Technology,State Key Laboratory of Meat Quality Control and Cultured Meat Development,Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province,Jiangsu Collaborative Innovation Center of Meat Production and Processing,Quality and Safety Control,Nanjing Agricultural University,No.1 Weigang,Nanjing 210095,P.R.China
农业科技
Adipogenic progressionBMPERBroilersFibro/adipogenic progenitorssnRNA-seq
《Journal of Animal Science and Biotechnology》 2026 (4)
P.2377-2395,19
supported by the National Natural Science Foundation of China(32573161)the Fundamental Research Funds for the Central Universities(YDZX2023003)the Jiangsu Agriculture Science and Technology Innovation Fund(CX(23)1016)the Fundamental Research Funds for the Central Universities(QTPY2025013)。
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