The firsttelomere-to-telomere genome assembly of the soybean male-sterile germplasm 88-428BY by massive parallel sequencingOA
Soybean(Glycine max)is a key source of plant protein and oil,yet genomic resources for male-sterile germplasms remain scarce.This study addresses this gap by presenting the first telomere-totelomere(T2T)genome assembly of the landrace soybean 88-428BY,a Chinese photoperiod-sensitive genic male sterility(PGMS)germplasm with photoperiod-dependent fertility.Using a hybrid strategy combining PacBio HiFi,Oxford Nanopore ultra-long reads,and Hi-C scaffolding,we generated a highquality reference genome of 1.01 Gb(N50=52.05 Mb),achieving 99.88%BUSCO completeness and assembling telomeres at both ends of 80%of the chromosomes.Annotation revealed 55,292 proteincoding genes and a high repetitive content(63.03%),which was dominated by LTR retrotransposons.Comparative genomics identified35,236 structural variations(SVs),with hotspot regions harboring genes showing suppressed expression.Transcriptomic analysis under long-day and short-day conditions uncovered co-expression modules enriched in flavonoid biosynthesis and protease inhibitors,alongside key transcription factors(e.g.,NAC25,ERF113)potentially associated with fertility regulation.This T2T genome provides a critical resource for elucidating the molecular basis of photoperiod-sensitive male sterility and advancing functional genomics and molecular breeding in soybean.
Yuhua Yang;Zhiyuan Bai;Yan Chen;Mengen Nie;Haigang Wang;Haiping Zhang;Ruijun Zhang;Zhixin Mu
Center for Agricultural Genetic Resources Research,Shanxi Agricultural University,Taiyuan 030031,ChinaCenter for Agricultural Genetic Resources Research,Shanxi Agricultural University,Taiyuan 030031,ChinaCenter for Agricultural Genetic Resources Research,Shanxi Agricultural University,Taiyuan 030031,ChinaCenter for Agricultural Genetic Resources Research,Shanxi Agricultural University,Taiyuan 030031,ChinaCenter for Agricultural Genetic Resources Research,Shanxi Agricultural University,Taiyuan 030031,ChinaCenter for Agricultural Genetic Resources Research,Shanxi Agricultural University,Taiyuan 030031,ChinaCenter for Agricultural Genetic Resources Research,Shanxi Agricultural University,Taiyuan 030031,ChinaCenter for Agricultural Genetic Resources Research,Shanxi Agricultural University,Taiyuan 030031,China
农业科技
Glycine max88-428BYTelomere-to-telomereStructure variationsPhotoperiod treatment
《Plant Diversity》 2026 (4)
P.836-845,10
supported by Major Science and Technology Special Program of Shanxi Province(No.202201140601025)the Natural Science Foundation of Shanxi Province(No.202303021211085)the China Postdoctoral Science Foundation and Shanxi Agricultural University Outstanding Doctoral Startup Project(2023BQ95)。
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