Stratified profiling of azoospermia:Differentiating histological subtypes with seminal plasma metabolic signaturesOA
Non-obstructive azoospermia(NOA),characterized by impaired spermatogenesis and the complete absence of sperm in the ejaculate,represents one of the most severe forms of male infertility.Current approaches for definitive histological characterization and sperm retrieval often require invasive testicular procedures,underscoring the need for reliable,non-invasive methods for preoperative stratification.In the present study,we performed untargeted metabolomic profiling of human seminal plasma to identify biomarker panels capable of stratifying azoospermia subtypes through a stepwise approach.We identified distinct metabolite biomarker panels comparing NOA vs.obstructive azoospermia(OA),Sertoli cell-only syndrome vs.other NOA subtypes,and hypospermatogenesis vs.maturation arrest.Additionally,cross-species comparative analysis with high-resolution metabolomic data from purified mouse testicular cell populations implicated these biomarkers in specific germ cell stages and metabolic transitions during spermatogenesis.These findings provide a preliminary molecular framework for the non-invasive classification of azoospermia subtypes and warrant validation in larger independent cohorts.
Jiachen Wang;Tianyi Song;Hongqi Fan;Mengyuan Zhu;Ying Yao;Laihua Li;Mingxi Liu;Minjian Chen;Jiahao Sha;Xiaoyu Yang;Yan Yuan
State Key Laboratory of Reproductive Medicine and Offspring Health,Nanjing Medical University,Nanjing,Jiangsu 211166,ChinaState Key Laboratory of Reproductive Medicine and Offspring Health,Nanjing Medical University,Nanjing,Jiangsu 211166,ChinaDepartment of Endocrinology,the First Affiliated Hospital of Nanjing Medical University,Nanjing Medical University,Nanjing,Jiangsu 210029,ChinaKey Laboratory of Modern Toxicology of Ministry of Education,School of Public Health,Nanjing Medical University,Nanjing,Jiangsu 211166,ChinaState Key Laboratory of Reproductive Medicine and Offspring Health,Nanjing Medical University,Nanjing,Jiangsu 211166,ChinaState Key Laboratory of Reproductive Medicine and Offspring Health,Nanjing Medical University,Nanjing,Jiangsu 211166,ChinaState Key Laboratory of Reproductive Medicine and Offspring Health,Nanjing Medical University,Nanjing,Jiangsu 211166,ChinaKey Laboratory of Modern Toxicology of Ministry of Education,School of Public Health,Nanjing Medical University,Nanjing,Jiangsu 211166,ChinaState Key Laboratory of Reproductive Medicine and Offspring Health,Nanjing Medical University,Nanjing,Jiangsu 211166,ChinaState Key Laboratory of Reproductive Medicine and Offspring Health,Clinical Center of Reproductive Medicine,the First Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,ChinaState Key Laboratory of Reproductive Medicine and Offspring Health,Nanjing Medical University,Nanjing,Jiangsu 211166,China
医药卫生
non-obstructive azoospermiaseminal plasmametabolomicsdiagnostic biomarkers
《The Journal of Biomedical Research》 2026 (4)
P.425-435,I0017,12
funded by the National Key R&D Program(Grant Nos.2022YFC2702800 to Y.Yuan and 2021YFC2700501-1 to L.Li)the National Natural Science Foundation of China(Grant Nos.82221005 to J.Sha and 82201763 to L.Li)。
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