Mechanism of Buzhong Yiqi decoction(补中益气汤)for Hashimoto''s thyroiditis:insights from gut microbiota and metabolomicsOA
OBJECTIVE:To investigate the effect of Buzhong Yiqi decoction(补中益气汤BZYQ)and its potential mechanism by conducting a comprehensive analysis of intestinal microbiota and metabolomics in autoimmune thyroiditis(AIT)rats.METHODS:An AIT model with antibiotic cocktail were established.After 8 weeks of intervention with BZYQ,the levels of anti-thyroid peroxidase antibodies(TPOAb)and thyroglobulin antibodies(TGAb)were measured.Pathological changes were assessed using hematoxylin and eosin staining.Changes of gut microbiota and fecal metabolites were analyzed through 16S RNA sequencing and metabolomics analysis.RESULTS:BZYQ intervention improved pathological damage of thyroid gland in AIT rats and significantly reduced the levels of anti-thyroid antibodies.Additionally,it had an ameliorative effect on the pathological damage of the colon.Also,BZYQ increased the abundance of Lactobacillus reuteri,reversing the dysbiosis of the gut microbiota.And BZYQ could regulate tryptophan metabolism of tryptophan to reverse metabolic disorders in HT.The correlation analysis indicated a close relationship between tryptophan metabolism and Lactobacillus reuteri.CONCLUSIONS:BZYQ is a promising therapeutic approach for HT.Its mechanism in treating HT may be associated with the regulation of gut microbiota dysbiosis and the improvement of gut-derived metabolic disorders.
CHEN Ying;QIAO Jiajun;LU Yuyuan;XUE Chongxiang;XIA Zhongyuan
Graduate School,Beijing University of Chinese Medicine,Beijing 100029,China Surgery of Traditional Chinese Medicine,China-Japan Friendship Hospital,Beijing 100029,ChinaSurgery of Traditional Chinese Medicine,China-Japan Friendship Hospital,Beijing 100029,ChinaGraduate School,Beijing University of Chinese Medicine,Beijing 100029,China Surgery of Traditional Chinese Medicine,China-Japan Friendship Hospital,Beijing 100029,ChinaDepartment of Endocrinology,the Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310009,ChinaSurgery of Traditional Chinese Medicine,China-Japan Friendship Hospital,Beijing 100029,China
医药卫生
RNAribosomal16Ssequence analysisRNAgastrointestinal microbiomemetabolomicstryptophanBuzhong Yiqi decoction
《Journal of Traditional Chinese Medicine》 2026 (3)
P.571-583,13
National High Level Hospital Clinical Research Funding:Study on the Mechanism of the Gut Microbiota Participating in the Metabolic Regulation Therapy of Hashimoto''s Thyroiditis by Buzhong Yiqi decoction(2023-NHLHCRF-BQ-28)Tongchuan Thyroid Disease Prevention Center 2023 Research Special Youth Project:Study on the Mechanism of Buzhong Yiqi decoction in Treating Hashimoto''s Thyroiditis by Improving Gut Mucosal Barrier(TJF-QN-2023-06)National Natural Science Foundation of China:Action Mechanism of Buzhong Yiqi decoction in Treating Hashimoto''s Thyroiditis Based on Transforming Growth Factorβ/Smad Signaling Pathway and the Balance between Treg and Th17 Cells(No.81973855)。
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