Evaluation of the anti-apoptotic,anti-platelet aggregation,and proangiogenic properties of Naoshuantong capsule(脑栓通)in ischemic stroke:insights from network pharmacology and animal experimentsOA
OBJECTIVE:To explore the complex molecular mechanisms of Naoshuantong capsule(脑栓通,NST),a Traditional Chinese Medicine formula,against ischemic stroke(IS)using network pharmacology and various experimental verifications.METHODS:This study employed an integrative approach that combined network pharmacology with experimental validation.Specifically,a network pharmacology model was developed to clarify the fundamental mechanisms and essential NST targets against IS.The core targets and pathways were verified using molecular docking and animal experiments.Additionally,the predicted effects of NST were confirmed by a series of experimental techniques,including hematoxylin-eosin staining,platelet aggregation,flow cytometry,and Western blotting.RESULTS:A total of 299 targets for overlap of compounds-disease targets were predicted.Proteinprotein interaction(PPI)network analysis revealed that the top 30 core targets.Subsequent molecular docking analysis demonstrated that serine-threonine protein kinase 1(AKT1),phosphoinositide 3-kinase(PI3K),and vascular endothelial growth factor A(VEGFA)have significant binding abilities with these compounds.Gene ontology(GO)enrichment analysis indicated that NST mechanisms against IS involved anti-apoptosis,antiplatelet-aggregation,and angiogenesis ability,confirmed by animal experiments.GO pathway analysis further showed that the mechanism of NST in treating IS was associated PI3K-AKT signaling.Ultimately,this study determined that NST downregulated cleaved-caspase-3 expression while upregulated phosphorylated AKT(PAKT)/AKT,phosphatidylinositol-4,5-Bisphosphate 3-kinase/PI3K,B-cell lymphoma-2(Bcl-2)/Bcl-2-associated X protein,and VEGFA expressions.CONCLUSION:The analysis utilizing multiple approaches manifested that NST has anti-apoptotic,antiplatelet-aggregation,and pro-angiogenic properties in managing IS,offering a systemic outlook for investigating the impact of NST on IS.
LI Chuanpeng;YAO Yaoyao;XU Yingzhi;JIANG Ping;FENG Luda;GAO Ying;DONG Xinglu
Department of Neurology,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China Institute for Brain Disorders,Beijing University of Chinese Medicine,Beijing 100700,China Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100700,ChinaDepartment of Neurology,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China Institute for Brain Disorders,Beijing University of Chinese Medicine,Beijing 100700,China Chinese Medicine Key Research Room of Brain Disorders Syndrome and Treatment of the National Administration of Traditional Chinese Medicine,Beijing 100700,ChinaDepartment of Neurology,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China Institute for Brain Disorders,Beijing University of Chinese Medicine,Beijing 100700,China Chinese Medicine Key Research Room of Brain Disorders Syndrome and Treatment of the National Administration of Traditional Chinese Medicine,Beijing 100700,ChinaDepartment of Neurology,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China Institute for Brain Disorders,Beijing University of Chinese Medicine,Beijing 100700,China Chinese Medicine Key Research Room of Brain Disorders Syndrome and Treatment of the National Administration of Traditional Chinese Medicine,Beijing 100700,ChinaDepartment of Neurology,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China Institute for Brain Disorders,Beijing University of Chinese Medicine,Beijing 100700,China Chinese Medicine Key Research Room of Brain Disorders Syndrome and Treatment of the National Administration of Traditional Chinese Medicine,Beijing 100700,ChinaDepartment of Neurology,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China Institute for Brain Disorders,Beijing University of Chinese Medicine,Beijing 100700,China Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100700,China Chinese Medicine Key Research Room of Brain Disorders Syndrome and Treatment of the National Administration of Traditional Chinese Medicine,Beijing 100700,ChinaDepartment of Neurology,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China Institute for Brain Disorders,Beijing University of Chinese Medicine,Beijing 100700,China Chinese Medicine Key Research Room of Brain Disorders Syndrome and Treatment of the National Administration of Traditional Chinese Medicine,Beijing 100700,China
医药卫生
ischemic strokenetwork pharmacologyphosphatidylinositol 3-kinaseproto-oncogene proteins c-aktsignal transductionNaoshuantong capsule
《Journal of Traditional Chinese Medicine》 2026 (3)
P.629-640,12
Research on the Mechanism of Naoshuantong capsule in Treatment of Ischemic Stroke Based on Network Pharmacology(601249)the National Natural Science Foundation of China(Young Scientists Fund):“Study on the Effects and Mechanisms of Detoxification and Collateral-Unblocking Therapy on Ischemic Stroke by Regulating Mitochondria-associated ER membranes Ca2+Homeostasis via the Inositol 1,4,5-trisphosphate Receptor Type 1-Glucose-regulated Protein 75-Voltage-dependent Anion Channel 1 Complex”(No.82405317)Beijing Municipal level Traditional Chinese Medicine Expert Academic Experience Inheritance Work Project(202279)Research on the Phar macological Mechanism of Naoshuantong Capsule in the Treatment of Ischemic Stroke and Cerebral Small Vessel Disease(601418qt)。
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