Neuroprotective effects of shrimp peptides by improving synaptic plasticity through Adcy5/PKA/p-CREB based on proteomic techniquesOA
There is currently a great deal of interest in developing neuroprotective peptides to address memory impairment and cognitive decline.Although the shrimp peptide Gln-Met-Asp-Asp-Gln(QMDDQ)has been demonstrated to have neuroprotective activity by cellular assays,the molecular regulatory mechanism in animals remains unclear.In this study,we explored the neuroprotective effects of QMDDQ in mice with scopolamine-induced memory impartment using behavioral tests,tissue staining techniques,proteomics,immunofluorescence,and Western blotting.The results of the study showed that QMDDQ was able to shorten the escape latency,reduce the number of electric shocks,and improve the behavioral performance of mice.The neuronal damage in CA1,CA3 and Dentate Gyrus regions was better repaired,and QMDDQ also increased the content of acetylcholine in the hippocampus with the increase of the gavage dose,and the activity of acetylcholinesterase was inhibited.Proteomics data showed that a total of 62 differential proteins were identified in the model and QMDDQ groups,with KEGG pathway enrichment showed significant changes in 40 pathways such as the cAMP signaling pathway,as verified by the key protein adenylate cyclase 5(Adcy5).The results suggested that QMDDQ improved synaptic plasticity through the Adcy5/PKA/p-CREB pathway,thus exerting a neuroprotective effect protective effect.
Ruowen Liu;Jie Ding;Limin Zhong;Songyi Lin
SKL of Marine Food Processing&Safety Control,National Engineering Research Center of Seafood,School of Food Science and Technology,Dalian Polytechnic University,Dalian 116034,China Engineering Research Center of Special Dietary Food of Liaoning Province,Food Engineering Technology Research Center of Liaoning Province,Dalian 116034,ChinaSKL of Marine Food Processing&Safety Control,National Engineering Research Center of Seafood,School of Food Science and Technology,Dalian Polytechnic University,Dalian 116034,China Engineering Research Center of Special Dietary Food of Liaoning Province,Food Engineering Technology Research Center of Liaoning Province,Dalian 116034,ChinaGanzhou Quanbiao Biological Technology Co.,Ltd.,Ganzhou 341100,ChinaSKL of Marine Food Processing&Safety Control,National Engineering Research Center of Seafood,School of Food Science and Technology,Dalian Polytechnic University,Dalian 116034,China Engineering Research Center of Special Dietary Food of Liaoning Province,Food Engineering Technology Research Center of Liaoning Province,Dalian 116034,China Ganzhou Quanbiao Biological Technology Co.,Ltd.,Ganzhou 341100,China
医药卫生
Shrimp peptideNeuroprotectionHippocampusProteomicsSynaptic plasticity
《Food Science and Human Wellness》 2026 (6)
P.2905-2919,15
supported by the National Key Research and Development Program of China(2024YFD2101200)。
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