A biomimetic magnesium–hydrogel scaffold with ferroptosis regulatory function for bone defect repairOA
Segmental bone defects remain a major clinical challenge due to their poor self-healing capacity and disrupted local microenvironments.Ferroptosis,an iron-dependent form of regulated cell death driven by lipid peroxidation,has emerged as a critical barrier to bone regeneration under pathological conditions such as oxidative stress and iron overload.However,current grafting strategies lack the ability to modulate ferroptosis or adaptively regulate local stress responses.Here,we developed a biomimetic magnesium-hydrogel composite scaffold with ferroptosis regulatory capability.The scaffold integrates a three-dimensional(3D)-printed Mg framework,a dicalcium phosphate dihydrate(DCPD)coating for corrosion control,and a functional hydrogel composed of gelatin grafted with gallic acid(GA)and phosphocreatine(Pcr).This design synergistically slows degradation,buffers pH,and confers antioxidant and iron-chelating functions.In vitro and in vivo results demonstrated that the scaffold alleviates ferroptosis by upregulating GPX4 and SLC7A11,downregulating ACSL4 and TFR1,and activating MAPK/mTOR signaling,while promoting osteogenic differentiation and angiogenesis.Our strategy offers a multifunctional,microenvironment-responsive scaffold that addresses both structural and biological challenges in bone defect repair.
Jin Zhao;Haotian Qin;Bozun Miao;Peng Zhang;Hui Zeng;Deli Wang;Guangyin Yuan;Junyu Qian;Yingqi Chen
Department of Bone&Joint Surgery,National&Local Joint Engineering Research Center of Orthopaedic Biomaterials,Peking University Shenzhen Hospital,Shenzhen 518036,ChinaDepartment of Bone&Joint Surgery,National&Local Joint Engineering Research Center of Orthopaedic Biomaterials,Peking University Shenzhen Hospital,Shenzhen 518036,ChinaNational Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite School of Materials Science and Engineering,Shanghai Jiao Tong University,Shanghai,200240,ChinaDepartment of Bone&Joint Surgery,National&Local Joint Engineering Research Center of Orthopaedic Biomaterials,Peking University Shenzhen Hospital,Shenzhen 518036,ChinaDepartment of Bone&Joint Surgery,National&Local Joint Engineering Research Center of Orthopaedic Biomaterials,Peking University Shenzhen Hospital,Shenzhen 518036,ChinaDepartment of Bone&Joint Surgery,National&Local Joint Engineering Research Center of Orthopaedic Biomaterials,Peking University Shenzhen Hospital,Shenzhen 518036,ChinaNational Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite School of Materials Science and Engineering,Shanghai Jiao Tong University,Shanghai,200240,ChinaDepartment of Bone&Joint Surgery,National&Local Joint Engineering Research Center of Orthopaedic Biomaterials,Peking University Shenzhen Hospital,Shenzhen 518036,ChinaDepartment of Bone&Joint Surgery,National&Local Joint Engineering Research Center of Orthopaedic Biomaterials,Peking University Shenzhen Hospital,Shenzhen 518036,China Medical Innovation Technology Transformation Center of Shenzhen Second People’s Hospital,the First Affiliated Hospital,Shenzhen University,Shenzhen 518055,China
医药卫生
Anti-ferroptosisBone regenerationBiomimetic magnesium-hydrogel scaffoldCorrosion control.
《Journal of Magnesium and Alloys》 2026 (6)
P.350-367,18
supported by the Guangdong Basic and Applied Basic Research Foundation(No.2022B1515120046)Research and Development Projects of Shenzhen(No.JCYJ20220818102815033)Shenzhen Key Laboratory of Orthopaedic Diseases and Biomaterials Research(No.ZDSYS20220606100602005)China Postdoctoral Science Foundation(No.2024M752092)National Natural Science Foundation of China(Key Program:Grant No 52130104)National Key Research and Development Program of China(2024YFB4610104)Shenzhen Medical Research Fund(No.A2402040)Shenzhen Science and Technology Program(No.JCYJ20240813180905008)Sanming Project of Medicine in Shenzhen(No.SZSM202211038)the Shenzhen Portion of Shenzhen-Hong Kong Science and Technology Innovation Cooperation Zone(No.HTHZQSWS-KCCYB-2023060).
评论