Characterization of main strains of ARC inoculant via machine learning-assisted single-cell Raman spectroscopyOA
ARC inoculant(A,aflatoxin prevention and control;R,Rhizobia nodulation induction;C,Coupling)is a brandnew inoculant with coupling function that enhances legume quality and nitrogen fixation.Comprehensive characterization of its key functional strains is critical for establishing a quality-control framework for the inoculant''s formulation.Here,we constructed a characteristic spectral dataset comprising over 63,000 single-cell Raman spectra of the constituent strains by employing Ramanome technology.Six machine learning-based predictive models were developed and compared for the constituent strains,while the Linear Discriminant Analysis(LDA)model demonstrated the best performance,with a classification accuracy exceeding 92.4%.This work provides a unique spectral fingerprint for ARC inoculant and will directly aid its application in sustainable agricultural production.
Jie Kang;Feng Wei;Xi Shen;Ao Liu;Jingjing Dou;Shujie Zhang;Jinli Hu;Liangxiao Zhang;Qi Zhang;Peiwu Li
Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Laboratory of Risk Assessment for Oilseeds Products(Wuhan),Ministry of Agriculture and Rural Affairs,Quality Inspection and Test Center for Oilseed Products,Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Laboratory of Risk Assessment for Oilseeds Products(Wuhan),Ministry of Agriculture and Rural Affairs,Quality Inspection and Test Center for Oilseed Products,Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,ChinaInstitute of Food Safety,Hubei University,Wuhan 430061,ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Laboratory of Risk Assessment for Oilseeds Products(Wuhan),Ministry of Agriculture and Rural Affairs,Quality Inspection and Test Center for Oilseed Products,Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,ChinaCollege of Resources and Environment,Huazhong Agricultural University,Wuhan 430070,ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Laboratory of Risk Assessment for Oilseeds Products(Wuhan),Ministry of Agriculture and Rural Affairs,Quality Inspection and Test Center for Oilseed Products,Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,ChinaSchool of Resources and Environmental Engineering,Wuhan University of Technology,Wuhan 430070,ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Laboratory of Risk Assessment for Oilseeds Products(Wuhan),Ministry of Agriculture and Rural Affairs,Quality Inspection and Test Center for Oilseed Products,Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China Institute of Food Safety,Hubei University,Wuhan 430061,China Hubei Hongshan Laboratory,Wuhan 430070,ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Laboratory of Risk Assessment for Oilseeds Products(Wuhan),Ministry of Agriculture and Rural Affairs,Quality Inspection and Test Center for Oilseed Products,Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China Institute of Food Safety,Hubei University,Wuhan 430061,China Hubei Hongshan Laboratory,Wuhan 430070,ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Laboratory of Risk Assessment for Oilseeds Products(Wuhan),Ministry of Agriculture and Rural Affairs,Quality Inspection and Test Center for Oilseed Products,Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China Institute of Food Safety,Hubei University,Wuhan 430061,China Hubei Hongshan Laboratory,Wuhan 430070,China Xianghu Laboratory,Hangzhou 311231,China
农业科技
Symbiotic nitrogen fixationLegumeARC inoculantSingle-cell Raman spectraMachine learning modelsQuality-control framework
《Oil Crop Science》 2026 (2)
P.113-116,4
financially supported by the Special Funds of the National Natural Science Foundation of China(32441047)the Major Project of Hubei Province Science&Technology(2023BBA002)the“Pioneer”and“Leading Goose”R&D Program of Zhejiang(2024SSYS0103)the Major Scientific and Technological Tasks of the Chinese Academy of Agricultural Sciences(CAAS-ZDRW202416)。
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