Genetic dissection of salt tolerance architecture in Brassica napus at the seedling stage by genome-wide association studyOA
Owing to the continuous increase in industrialization,the extent of soil salinization is escalating globally.Brassica napus is among the most advantageous field crops for the development and utilization of saline-alkali land.Nevertheless,the molecular regulation of salt tolerance during the seedling stage in this species remains unclear.To explore this mechanism,an association population consisting of 202 accessions was subjected to a 257 mmol/L NaCl solution at the seedling(four-leaf)stage.After 14 days of salt treatment,the above-ground and under-ground fresh and dry weights of each accession line were measured,and the correlations between these traits were evaluated.By integrating phenotypic data with resequencing data,a genome-wide association study identified 2043 single nucleotide polymorphisms(SNPs)that were significantly associated with these traits.Ninety SNPs were detected repeatedly,with a single SNP accounting for 1.3%–5.29%of the phenotypic variation.Subsequently,two candidate genes were identified,and six germplasm resources with strong salt tolerance at the seedling stage were selected.These results will guide strategies for breeding salt-tolerant B.napus and will offer a theoretical foundation for the restoration and management of salinized land.
Tinghai Yang;Wei Zhang;Yue Shen;Feng Chen;Chunyun Wang;Xiaoying Zhou;Chengming Sun;Sanxiong Fu;Jiefu Zhang;Xiaodong Wang
Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Key Laboratory of Cotton and Rapeseed,Ministry of Agriculture and Rural Affairs,Nanjing,210014,ChinaInstitute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Key Laboratory of Cotton and Rapeseed,Ministry of Agriculture and Rural Affairs,Nanjing,210014,ChinaInstitute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Key Laboratory of Cotton and Rapeseed,Ministry of Agriculture and Rural Affairs,Nanjing,210014,ChinaInstitute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Key Laboratory of Cotton and Rapeseed,Ministry of Agriculture and Rural Affairs,Nanjing,210014,ChinaInstitute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Key Laboratory of Cotton and Rapeseed,Ministry of Agriculture and Rural Affairs,Nanjing,210014,ChinaInstitute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Key Laboratory of Cotton and Rapeseed,Ministry of Agriculture and Rural Affairs,Nanjing,210014,ChinaInstitute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Key Laboratory of Cotton and Rapeseed,Ministry of Agriculture and Rural Affairs,Nanjing,210014,ChinaInstitute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Key Laboratory of Cotton and Rapeseed,Ministry of Agriculture and Rural Affairs,Nanjing,210014,ChinaInstitute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Key Laboratory of Cotton and Rapeseed,Ministry of Agriculture and Rural Affairs,Nanjing,210014,ChinaInstitute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Key Laboratory of Cotton and Rapeseed,Ministry of Agriculture and Rural Affairs,Nanjing,210014,China
农业科技
Brassica napusSalt toleranceGenome-wide association studySeedling stageSingle nucleotide polymorphismCandidate geneGermplasm resource
《Oil Crop Science》 2026 (2)
P.138-149,12
supported by Biological Breeding-National Science and Technology Major Project(2022ZD04010)Jiangsu Provincial Frontier Technology R&D Program(Modern Agriculture)(BF2025305)Jiangsu Agricultural Science and Technology Innovation Fund(CX(24)1003)China Agriculture Research System of MOF and MARA(CARS-12)。
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