Engineered Lactobacillus reuteri for scavenging reactive oxygen species and modulating oral microflora in periodontitis therapyOA
The onset and progression of periodontitis are closely associated with subgingival dysbiosis and excessive localized oxidative stress.While some oral probiotics exhibit certain inhibitory effects on periodontitis-related pathogens,they often struggle to effectively colonize and antagonize these pathogens due to the complex oxidative stress at the site of periodontitis.In this study,we engineer Lactobacillus reuteri with a reactive oxygen species(ROS)-responsive adhesive polymer(phenylboric acid-dopamine-hyaluronic acid)(LR@PDH).In the periodontitis microenvironment,this polymer can consume ROS and then expose the phenolic hydroxyl group of dopamine,promoting the selective adhesion and colonization of Lactobacillus reuteri at the site of inflammation to antagonize pathogens.The results show that,compared to conventional probiotic therapy,inflammation-responsive adhesive Lactobacillus reuteri effectively alleviates local oxidative stress,reduces the abundance of pathogenic bacteria in the subgingival microbiome,and inhibits the progression of periodontitis.Additionally,its good biocompatibility and safety highlight its potential as a therapeutic approach for clinical treatment of periodontitis.
Yuqiang Wang;Ying Tang;Qianxiao Huang;Jiaxin An;Yueli Zhou;Hongye Yang;Fangfang Song;Xianzheng Zhang;Cui Huang
State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration&Key Laboratory of Oral Biomedicine Ministry of Education&Hubei Key Laboratory of Stomatology&School&Hospital of Stomatology,Wuhan University,Wuhan,ChinaState Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration&Key Laboratory of Oral Biomedicine Ministry of Education&Hubei Key Laboratory of Stomatology&School&Hospital of Stomatology,Wuhan University,Wuhan,ChinaKey Laboratory of Biomedical Polymers of Ministry of Education&Department of Chemistry,and Institute for Advanced Studies,Wuhan University,Wuhan,ChinaKey Laboratory of Biomedical Polymers of Ministry of Education&Department of Chemistry,and Institute for Advanced Studies,Wuhan University,Wuhan,ChinaState Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration&Key Laboratory of Oral Biomedicine Ministry of Education&Hubei Key Laboratory of Stomatology&School&Hospital of Stomatology,Wuhan University,Wuhan,ChinaState Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration&Key Laboratory of Oral Biomedicine Ministry of Education&Hubei Key Laboratory of Stomatology&School&Hospital of Stomatology,Wuhan University,Wuhan,ChinaState Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration&Key Laboratory of Oral Biomedicine Ministry of Education&Hubei Key Laboratory of Stomatology&School&Hospital of Stomatology,Wuhan University,Wuhan,ChinaKey Laboratory of Biomedical Polymers of Ministry of Education&Department of Chemistry,and Institute for Advanced Studies,Wuhan University,Wuhan,ChinaState Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration&Key Laboratory of Oral Biomedicine Ministry of Education&Hubei Key Laboratory of Stomatology&School&Hospital of Stomatology,Wuhan University,Wuhan,China
医药卫生
oxidative stresswhileoral probioticsreactive oxygenreactive oxygen speciesengineered lactobacillus reuteriengineer lactobacillus reuterisubgingival dysbiosisoral microflora
《International Journal of Oral Science》 2026 (3)
P.488-503,16
financial support by the National Natural Science Foundation of China(82301154,82471018,and 82271010)The Interdisciplinary Research Project of School of Stomatology Wuhan University(XNJC202307)。
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