Tracheal nebulization of AAV-CRE for lung conditional knockout model construction and whole lung clearingOA
The development of lung-specific conditional knockout animal models is pivotal in advancing lung disease research.This protocol describes the experimental procedures in detail,including the construction of lox P mice,endotracheal tube nebulization of adeno-associated virus(AAV)and whole lung clearing.Mouse lung conditional knockouts were successfully constructed.This meticulous approach allows for accurate control over the timing and location of the knockout,facilitating an in-depth investigation into the pathogenesis of lung diseases and the development of therapeutic strategies.These lung-specific conditional knockout models represent a powerful tool for the study of lung diseases,offering valuable insights and guidance for the advancement of treatment approaches and drug discovery in the future.
Lihong Ye;Xiyue Yan;Dacheng Yang;Shuang Wu;Wandong Chen;Wei Kevin Zhang
Graduate School of Guangzhou Medical University,Guangzhou 511436,China Guangzhou National Laboratory,Guangzhou 510005,ChinaGuangzhou National Laboratory,Guangzhou 510005,ChinaGraduate School of Guangzhou Medical University,Guangzhou 511436,China Guangzhou National Laboratory,Guangzhou 510005,ChinaGraduate School of Guangzhou Medical University,Guangzhou 511436,China Guangzhou National Laboratory,Guangzhou 510005,ChinaGuangzhou National Laboratory,Guangzhou 510005,ChinaGraduate School of Guangzhou Medical University,Guangzhou 511436,China Guangzhou National Laboratory,Guangzhou 510005,China
医药卫生
Lung condition knockoutAAVCre-loxP mouse modelTracheal nebulizationWhole lung clearing
《Biophysics Reports》 2026 (3)
P.151-161,11
supported by grants from the Guangzhou National Laboratory(YW-YWYM0204,GZNL2023A01008)the National Key Research and Development Program of China(2021YFA1101304)。
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