Network pharmacology-based strategy to reveal how Qingwen Baidu decoction protects against LPS-induced lung injury both in vitro and in vivoOA
Background:Acute lung injury(ALI),a critical respiratory condition,represents a significant global health challenge due to its high incidence rates around the world.Qingwen Baidu Decoction(QWBD)is a classical formula with anti-inflammatory effects and antioxidant characteristics.It plays an integral part in infectious disease management.However,the mechanism by which QWBD combats ALI remains markedly unexplored.In order to clarify the preventive efficacy and underlying mechanisms of QWBD in ALI,this study utilized a multi‑approach strategy combining network pharmacology,molecular docking,and in vitro/in vivo studies was adopted.Methods:Network pharmacology was utilized to search for bioactive components,key targets and signaling pathways of QWBD against ALI.Molecular docking served as a key method to verify how the principal active monomers in QWBD bind to the identified core targets.Ultra-performance liquid chromatography(UPLC)was used to characterize the chemical compounds of QWBD.Next,we developed both a RAW264.7 cell injury model and a rat model of ALI using LPS.Finally,the effectiveness and mechanism of QWBD in ALI were demonstrated through combination of in vivo/in vitro studies.Results:The network pharmacology results revealed 221 principal bioactive compounds and 217 overlapping core targets between QWBD and ALI.According to GO and KEGG enrichment analyses,QWBD''s mechanism of action appears to involve the modulation of genes related to inflammation,apoptosis,and oxidative stress,along with the TLR4-JNK and PI3K-Akt pathways.Among the QWBD compounds,quercetin,kaempferol,β-sitosterol,luteolin,and wogonin were present in high concentrations.Molecular docking analysis confirmed the strong interaction between the five bioactive components and their corresponding targets docking targets.The results of these experiments confirmed that QWBD markedly alleviated ALI via its ability to ameliorate pulmonary edema,inflammatory cell infiltration,as well as cytokine levels while restoring alveolar structural integrity.Conclusion:QWBD exerts its protective effects by modulating the PI3K/Akt and TLR4/JNK signaling pathway.Overall,QWBD protects against LPS-induced ALI,highlighting its potential as a novel preventive agent.
Wen-Ting Wang;Yan-Ling He;Jin-Hui Feng;Ke Yang;Sha Li;Bin Wang;Ji-Ping Liu;Yong-Heng Shi;Hao Wei;Chuan Wang;Peng Zhao;Tai-Wei Dong;Fei Luan;Shuan-Lao Yang;Guo-Quan Wang
Pharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,China Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,China Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,China Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,China Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang 712046,ChinaThe Second Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang,Shaanxi 712000,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,China Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,China Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,China Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,China Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,China Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,China Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang 712046,China College of Clinical Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,China
医药卫生
acute lung injurytraditional chinese medicineanti-inflammatoryantioxidantexperimental validation
《Traditional Medicine Research》 2026 (12)
P.30-41,12
supported by the Natural Science Foundation of Shaanxi Province(Grant No.2021JM-470)the Administration of Traditional Chinese Medicine of Shaanxi Province(Grant No.2025-CXTD-04)the Educational Commission of Shaanxi Province,China(Grant No.24JR051).
评论