基于网络药理学、分子对接和实验验证索罗西汤颗粒预防慢性阻塞性肺疾病的作用及机制研究OA

Objective:To preliminarily investigate the pharmacodynamics and mechanism of Tibet medicine classic formula Srolo Bzhtang(SBT)granules in preventing chronic obstructive pulmonary disease(COPD)based on network pharmacology,molecular dock-ing,and in vivo experiments.Methods:The intersection of SBT blood components and COPD disease targets was screened,followed by network construction and enrichment analysis.Key targets were validated using molecular docking.Rats were divided into blank control group,model group,dexamethasone group,and SBT low-,medium-,and high-dose groups.The COPD model was established using cigarette smoke combined with lipopolysaccharide in all groups except the control group.Preventive administration was adopted,with the dexamethasone group(0.2 g·kg-1),SBT low-dose group(1.35 g·kg-1),SBT medium-dose group(2.7 g·kg-1),and SBT high-dose group(8.1 g·kg-1),each consisting of 10 rats.All groups received once-daily intragastric administration for 60 days,while the con-trol group and model group received an equal volume of normal saline.Lung histopathology and collagen deposition were observed.Se-rum levels of inflammatory factors(TNF-α,IL-iβ and IL-6)and oxidative stress markers(MDA and SOD)were measured.Expression of MAPK and PI3K/Akt pathway-related proteins was detected by Western blot.Results:A total of 125 intersecting targets were identi-fied between SBT blood components and COPD,with key enrichment pathways including MAPK and PI3K-Akt.Animal experiments showed that compared with the model group,each SBT dose group reduced TNF-α、1L-1β、1L-6 levels,increased SOD activity,de-creased MDA content,and downregulated the protein expression of p-ERK,p-PI3K,p-AKT,p-p38,and p-JNK in lung tis-sue.Conclusion:SBT granules exert anti-inflammatory and anti-oxidative stress effects by simultaneously regulating the PI3K/AKT and MAPK signaling pathways,representing a key mecha-nism in its prevention of COPD.