颅复丸对小鼠的急性毒性研究OA

Objective:To investigate the acute toxicity of Lufu Pills(LFP)in Kunming(KM)mice.Methods:Forty-eight KM mice were randomly divided into three groups:a blank control group and high-and low-dose LFP groups.The control group received normal saline,whereas the treatment groups received LFP at 13.2 and 6.6 g·kg-1,respectively,via gavage.All animals were observed for 14 consecutive days,during which general condition,body weight and behavioral changes were recorded.At the end of the observation period,serum samples were collected for routine biochemical analyses.The mice were then necropsied.Liver,heart,spleen,lung and kidney were examined macroscopically,weighed to calculate organ coefficients,and processed for histopathological evaluation.Results:No deaths or overt signs of toxicity occurred in any group.No significant differences were observed among groups in body weight,organ coefficients,or histopathological findings.Although biochemical parameters such as ALT(alanine aminotransferase),TP(total protein),ALB(albumin),TG(triglycerides),and UREA(urea nitrogen)showed vari-able elevations,these changes lacked a clear dose-dependent relationship.Conclusion:Within the dose range of 13.2 g·kg-1,intra-gastric administration of LFP did not cause significant pathological damage to the major organs of KM mice.However,it is neces-sary to monitor its impact on liver and kidney functions during the medication process.This study can provide a reference for clini-cal medication.