痛风定胶囊对高尿酸血症大鼠的降尿酸及肝肾保护作用OA

Objective:To explore the protective effect of Tongfengding capsule(TFDC)against hyperuricemia(HUA)and liver and kidney injuries in rats.Methods:The HUA rat model was established by intragastric administration of ethambutol combined with adenine.Meanwhile,TFDC(164.4,493.3,1 479.9 mg·kg-1)were given by intragastric administration for seven days.After the experiment,the kidney indexes were calculated,and the levels of uric acid(UA),creatinine(CRE),blood urea nitrogen(BUN),aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in the serum of rats in each group were determined.Additionally,the activities of xanthine oxidase(XOD)in the liver tissue were measured.The pathological charac-teristics of liver and kidney tissues were observed using hematoxylin-eosin(HE)staining method.Results:In model rats,se-rum UA,BUN,CRE,AST,ALT levels,hepatic XOD activity and renal coefficient increased markedly,accompanied by renal tubular dilatation and glomerular atrophy in kidney,as well as inflammatory cell infiltration,mild fatty degeneration and edema of hepatocytes in liver.Medium-and high-dose TFDC significantly reduced the renal coefficient,serum UA and BUN and CRE levels(P＜0.05,P＜0.01 or P＜0.001).The high-dose group markedly decreased serum AST(P＜0.05).While All doses of TFDC groups effectively lowered serum ALT(P＜0.05,P＜0.01 or P＜0.001),pathological lesions of liver and kidney in HUA rats.Con-clusion:TFDC can effectively reduce the serum UA level in HUA rats,which may be associated with the inhibition of XOD ac-tivities in liver.Meanwhile,the preparation provides favorable protective effects against hepatic and renal injury induced by HUA,which offers experimental evidence for expanding its clinical indications.