淫羊藿次苷Ⅱ的抗炎活性及机制研究OA

Objective:To clarify the anti-inflammatory activity of icariside Ⅱ from EpimedⅡ Folium and reveal its underly-ing mechanism,so as to provide experimental evidence for the anti-inflammatory pharmacodynamic material basis of EpimedⅡFolium and the research and development of innovative drugs.Methods:An in vitro inflammatory model was established by in-ducing mouse RAW 264.7 macrophages with lipopolysaccharide(LPS).Blank control group,model control group,positive con-trol group,and low-,medium-and high-dose groups of icariside Ⅱ were set up.The release level of nitric oxide(NO)in cell super-natant was detected using a nitric oxide assay kit.The mRNA expression levels of inflammatory factors IL-1β,IL-6 and TNF-αwere detected by quantitative real-time PCR(RT-qPCR).The release of nitric oxide(NO)was measured by Griess reagent assay.The protein expression of inducible nitric oxide synthase(iNOS),cyclooxygenase-2(COX-2)and NF-κB pathway-related proteins was determined by Western blot.Results:All tested doses of icariside Ⅱ markedly downregulated the mRNA expression levels of IL-1β,IL-6 and TNF-α in LPS-stimulated RAW 264.7 murine macrophages,suppressed NO production in a dose-dependent man-ner,and significantly reduced the protein expressions of iNOS and COX-2,as well as the expression of p-IKK,p-IκB and p-p65.Conclusion:Icariside Ⅱ exerts a significant inhibitory effect on LPS-induced inflammatory responses in RAW 264.7 cells.Its mechanism may be related to the regulation of NF-κB signaling pathway,the inhibition of inflammatory factor release,and the downregulation of iNOS and COX-2 protein expression.