不同产区甘草药材遗传特征与品质关键驱动因子研究OA

Objective:To clarify the botanical origin and population genetic diversity of Glycyrrhizae Radix et Rhizoma from different production areas and analyze the key factors affecting the accumulation of active components,thereby providing a basis for standardized cultivation and quality control of Glycyrrhizae Radix et Rhizoma.Methods:Inter-simple sequence repeat(ISSR)molecular markers were employed to analyze the genetic characteristics of the samples.DNA barcoding was used for the identification of botanical origin.The content of eight active components in Glycyrrhizae Radix et Rhizoma was determined by high performance liquid chromatography(HPLC).The data of 19 climatic factors were obtained from the Geographic Information System for Global Medicinal Plants(GMPGIS).Mantel correlation analysis and grey relational analysis were performed to reveal the effects of genetic factors and climatic factors on the accumulation of active components in Glycyrrhizae Radix et Rhizoma from different botanical origins.Results:The tested samples belonged to two species:Glycyrrhiza uralensis and G.glabra.The populations from Gansu and Inner Mongolia exhibited rich genetic diversity.Climatic factors such as temperature and precipitation,as well as genetic parameters including the number of alleles and gene diversity index,showed positive correlations with the content of active components in Glycyrrhizae Radix et Rhizoma.Climatic factors were the dominant factors affecting the accumulation of active components in G.uralensis,whereas genetic factors mainly influenced the accumulation of active components in G.glabra.Conclusion:This study clarifies the botanical origin of Glycyrrhizae Radix et Rhizoma from different production areas and the effects of genetic and climatic factors on the active component content of the two species,providing a methodological reference for quality evaluation and geoherbalism research of Glycyrrhizae Radix et Rhizoma.