向日葵 Trihelix 转录因子家族鉴定及表达分析OA

Trihelix transcription factors play important roles in plant growth,development,and various stress responses.However,few studies have been conducted on the systematic identification and expression characteristics of trihelix gene family in sunflower(Helianthus annuus L.).In this study,based on bioinformatics methods,the trihelix genes in sunflower genome were identified and characterized,and chromosomal localization,duplication events,phylogenetic analysis,gene structures,conserved motifs and expression patterns under stress conditions were analyzed.The results showed that a total of 46 trihelix genes were identified in the sunflower genome,unevenly distributed across 15 chromosomes.Among them,three pairs of tandemly duplicated genes and six pairs of segmentally duplicated genes were detected.According to phylogenetic analysis,sunflower trihelix family was divided into five subfamilies,and the members of the same subfamily have similar gene structures and conserved motifs.Thirty-six sunflower trihelix genes were expressed in all five organs(root,stem,leaf,flower and bud).Based on transcriptome sequencing(RNA-seq)data,29 trihelix genes significantly responded to Orobanche cumana Wallr.stress.