miRNA-1907在肾纤维化的表达及机制研究OA

Objective Based on bioinformatics and experimental verification methods,the expression changes and potential mechanisms of microRN(miRNA)-1907 in renal fibrosis were explored.Methods Two renal fibrosis miRNA expression profile datasets(GSE162794 and GSE42716)were procured from the Gene Expression Omnibus(GEO)database.GEO2R was employed to screen differentially expressed miRNAs.The expression level of miRNA-1907 in a renal fibrosis mouse model was validated via real time fluorescent quantitative polymerase chain reaction.The prediction of target genes for miRNA-1907 was facilitated by Starbase v3.0.The STRING database was utilised to construct protein-protein interaction(PPI)networks.The Cytoscape 3.8.2 software was utilised to construct competitive endogenous RNA(ceRNA)regulatory networks and to identify Hub genes.The functional enrichment analysis was performed using the R software.Results Three differentially expressed miRNAs(namely,miRNA-1907,miRNA-714 and miRNA-770-3p)as upregulated,with miRNA-1907 exhibiting the most pronounced differential expression.The verification experiment results showed that the expression level of miRNA-1907 in the kidneys of mice with renal fibrosis was significantly increased.In addition,analysis of the ceRNA regulatory network revealed that miRNA-1907 interacted with 278 messenger RNAs and 35 long non-coding RNAs.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses indicated that miRNA-1907 target genes were significantly enriched in biological processes and signalling pathways,including positive regulation of cell development,positive regulation of protein kinase activity,the phosphoinositide 3-kinase/protein kinase B signalling pathway,and cellular senescence.A total of nine Hub genes were identified through the process of PPI network analysis.These genes were found to be significantly involved in a variety of biological processes,including transferase complexes,lipid phosphorylation,external components of the membrane,inositol lipid-mediated signalling,insulin response,phosphatidylinositol metabolism.Conclusion The significant upregulation of miRNA-1907 in renal fibrosis may be involved in the process of renal fibrosis by regulating the ceRNA network and related signaling pathways.