侵染云南省滇红花的黄瓜花叶病毒的基因组特征及致病性分析OA

Cucumber mosaic virus(CMV)belongs to the genus Cucumovirus in the family Bromoviridae and has a wide host range,infecting 1 071 species in 521 genera across 100 families.Carthamus tinctorius(safflower)is an important medicinal plant,and CMV is widely prevalent in safflower worldwide,causing severe damage.In this study,safflower plants exhibiting symptoms of chlorosis,dwarfing,and bud blight were collected in Yunnan province for virus detection.Through small RNA sequencing and RT-PCR amplification,three CMV-infcctcd plants were identified,and the complete genome sequences of CMV RNA1,RNA2,and RNA3 were obtained and designated as CMV-crocus.Sequence analysis showed that the 2b protein of CMV-crocus differed greatly from those of other CMV isolates.Further investigations revealed that satellite RNAs from other isolates could reduce the viral accumulation of CMV-crocus in safflower.This study is the first to obtain the full-length genome sequence of CMV from safflower in Yunnan and to investigate its pathogenicity,providing a basis for subsequent virus control and prevention in safflower.