本氏烟肌球蛋白NbMyosin Ⅺ-2调控番茄黄化曲叶病毒细胞间运动的分子机制OA

Tomato yellow leaf curl virus(TYLCV)is a globally distributed plant virus that causes severe damage to crops such as tomato and tobacco plants.To investigate its pathogenic mechanisms,the TYLCV-encoded movement protein V2 was used as bait to screen and identify interacting protein from Nicotiana benthamiana.A myosin motor protein,NbMyosin Ⅺ-2(GenBank:DQ875135.1),was identified as a V2-interacting partner.Bimolecular fluorescence complementation,co-localization,and co-immunoprecipitation assays confirmed that V2 directly interacts with NbMyosin Ⅺ-2,and that they form punctate complexes along actin filaments.Gene silencing assays revealed that knockdown of NbMyosin Ⅺ-2 inhibited TYLCV infection in N.benthamiana and disrupted the localization of the TYLCV movement complex V2-C5 to plasmodesmata,trapping it in the cytoplasm,without affecting the silencing suppressor activity of V2.Furthermore,transient overexpression of a dominant-negative mutant of NbMyosin Ⅺ-2 significantly inhibited infection by TYLCV,tobacco curly shoot virus,and tomato leaf curl Yunnan virus.This study indicates that NbMyosin Ⅺ-2 regulates the intercellular movement of geminiviruses through interaction with V2,providing a potential target for the development of antiviral strategies.