花生籽仁品质性状与种皮颜色遗传分析及基因定位研究OA

Enhancing peanut kernel quality is crucial to ensuring grain and oil security and industrial competitiveness,with seed coat color being an important agronomic trait in peanut.In this study,a recombinant inbred line(RIL)population was constructed using Jihuatian 1(red seed coat)and JM135(pink seed coat)as parents.Major quality traits of the population kernels were de-tected using a near-infrared spectrometer.Based on HSV and RGB color space models,the RGB phenotypic data and seed coat color index(p)of the population were obtained.Genetic analysis was performed using the R package SEA v2.0.The results showed that the optimal genetic model for anthocyanin,oil,and sucrose contents in peanut kernels was 4MG-EEEA,for protein and linoleic acid contents,it was 4MG-EEA;and for oleic acid and palmitic acid contents,it was 4MG-CEA.The optimal genetic models for the R,G,B,and p values of peanut seed coat color were all 4MG-EEEA.Furthermore,the gene controlling seed coat color were preliminarily mapped using BSA-seq technology.By employing the ΔSNP-index method and the ED method,the tar-get gene was ultimately located in a 2.06 Mb interval on Arahy.03.Six candidate genes were screened based on gene annotation information.Expression profile and variation analysis indicated that Arahy.07ZIFT underwent a non-synonymous mutation,while Arahy.W8TDEC and Arahy.WZ366M exhibited high expression levels during seed development,suggesting they are likely candi-date genes associated with testa color.The research findings provide a theoretical basis for understanding the genetics and mo-lecular mechanisms of quality traits and seed coat color in peanuts.