菟丝子能减少阿尔茨海默病模型细胞的氧化损伤OA

Objective:To investigate the protective effect of Cuscuta chinensis Lam.against oxidative injury in an Alzheimer's disease(AD)cell model,as well as the related molecular mechanism.Methods:Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry was used to analyze and identify the chemical components of Cuscuta chinensis Lam..An oxidative injury model was established in HT22 neuronal cells treated with Aβ1-42.Cell viability was measured by CCK-8 assay,and apoptosis was detected by Annexin V-FITC/PI double staining.Intracellular reactive oxygen species(ROS)levels were detected using the DCFH-DA probe,and the expression of antioxidant-and apoptosis-related proteins was examined by Western blotting.Results:A total of 381 chemical components were identified from Cuscuta chinensis Lam.,among which astragalin,hyperoside,kaempferol,and quercetin were the main constituents.Cell experi-ments showed that Cuscuta chinensis Lam.at concentrations of 0.50,0.75,and 1.00 mg/mL increased the viability of Aβ1-42-treated HT22 cells,decreased the apoptosis rate,inhibited ROS excessive accumulation,upregulated the expression of the antioxidant-related proteins heme oxygenase-1 and NQO1,downregulated the expression of the apoptosis-related protein cytochrome C,and reduced the ratios of activated caspase-3/caspase-3 and BAX/Bcl-2.Conclusion:Cuscuta chinensis Lam.may attenuate oxidative injury in AD model cells by activating the endogenous antioxidant defense system and inhibiting the mitochondrial-dependent apoptotic pathway.