吲哚-3-丙酸通过C/EBP同源蛋白基因介导的凋亡通路缓解猪δ冠状病毒感染诱导的肠上皮损伤OA

Porcine deltacoronavirus(PDCoV)infection can cause severe enteritis in piglets,leading to high mortality rates.This study systematically screened indole metabolites with the potential to alleviate PDCoV-associated endoplasmic reticulum stress(ERS)and conducted an investigation into their mechanisms of action.The results showed that PDCoV infection could induce ERS and apoptosis in intestinal epithelial cells.Among various indole compounds,indole-3-propionic acid(IPA)demonstrated a significant inhibitory effect on ERS and concurrently reduced the level of apoptosis.Further validation in porcine small intestinal organoids and mouse infection models confirmed that IPA could specifically inhibit ERS and its mediated apoptosis in duodenal epithelial cells.In conclusion,this study demonstrates that IPA can alleviate PDCoV-induced intestinal epithelial damage by modulating the ERS pathway,providing a new theoretical foundation and empirical support for developing microbiota metabolite-based nutritional intervention strategies against enteric coronaviruses.