向日葵不同器官响应列当寄生的转录组分析OA

The damage caused by Orobanche parasitism on Helianthus annuus has been increasing year by year,severely restricting the improvement of sunflower yield and quality.To elucidate the molecu-lar regulatory mechanisms and metabolic pathways underlying the response of Helianthus annuus to Orobanche parasitism,the locally dominant cultivar SH361 was used as the experimental material.An Orobanche-infected group and a normal growth control group were established,and transcriptome se-quencing(RNA-seq)combined with bioinformatics analysis was employed to investigate the gene ex-pression patterns in 5 organs:roots,stems,leaves,petals,and seeds.The results showed that a total of 219.24 Gb of clean data was obtained,with GC contents ranging from 44.34％to 45.47％,and Q20 and Q30 base percentages not lower than 98.06％and 94.10％,respectively,indicating high sequen-cing data quality.Comparative analysis identified 1 531,2 314,2 290,2 137,and 1 658 differentially expressed genes(DEGs)in the respective organs,with the fewest DEGs in roots and the most in stems.KEGG pathway enrichment analysis of the DEGs revealed that the MAPK signaling pathway-plant was the core enriched pathway,and the pathway enrichment characteristics differed markedly a-mong organs.These DEGs synergistically mediate the defense response of Helianthus annuus to Oro-banche parasitism by regulating mRNA transcription and the biosynthesis and metabolism of resist-ance-related hormones.