贵州水稻抗稻瘟病基因分析及育种应用研究OA

To identify the major rice blast resistance genes in Guizhou rice germplasm,16 rice(Oryza sativa L.)germplasm resources were used as materials for field natural induction of rice blast resist-ance identification in Meitan,Guizhou,and functional fluorescent molecular markers for nine rice blast resistance genes(Pi2,Pi9,Pigm,Pi5,Pib,Pit,Pita,Pi33,and Pi46)were detected.The results showed that among the 16 rice germplasm resources,the rice blast resistance levels were as follows:seven highly susceptible,one susceptible,two moderately susceptible,three moderately re-sistant,and three resistant.Except for Pi9 and Pit,which were not detected,the remaining seven rice blast resistance genes were all detected.Combined with field phenotypic resistance identification and molecular marker detection of rice blast genes,materials carrying the rice blast resistance gene Pigm exhibited relatively strong rice blast resistance in the field.