基于转录组测序的人参成花基因挖掘及激素关联分析OA

To investigate the differentially expressed genes(DEGs)and endogenous hormone changes associated with flowering in Panax ginseng and to identify key regulatory factors,leaves from flow-ering and non-flowering plants of 3 Panax ginseng lines were used as materials.High-throughput transcriptome sequencing was employed for comparative transcriptomic analysis,endogenous hor-mone contents were determined using liquid chromatography-tandem mass spectrometry(LC-MS/MS),and the expression of key genes was validated by qRT-PCR.A total of 63 819 genes were ob-tained,and 2 365 DEGs related to flowering were identified.GO and KEGG enrichment analyses showed that the DEGs were significantly enriched in pathways such as secondary metabolite biosyn-thesis and plant hormone signal transduction.WGCNA identified a blue core module that was signifi-cantly positively correlated with gibberellin and cytokinin.PPI network analysis identified LFY,HTR2,and F24B9.25 as core hub genes in the flowering regulatory network.The qRT-PCR valida-tion results were consistent with the transcriptome sequencing data.This study confirmed that genes such as LFY,HTR2,and F24B9.25 were upregulated in flowering plants of Panax ginseng,revea-ling the potential key roles of gibberellin and cytokinin signaling pathways in the regulation of flower-ing in Panax ginseng.