藏药材西藏千里光种子萌发及组织培养研究OA

To establish a rapid propagation system for the Tibetan medicinal plant Senecio tibeticus,seed germination and seedling tissue culture of Senecio tibeticus were investigated separately.Seeds of Senecio tibeticus were treated with different disinfection methods,followed by soaking in different concentrations(1％,2％,3％)of H2O2,and then cultured on different media to observe seed germi-nation.The results showed that seeds of Senecio tibeticus were most effectively sterilized by treatment with 0.1％HgCl2 solution for 5 min.The average germination percentage in the indoor germination chamber was 81.6％,while the outdoor average was 57.3％,Higher concentrations(2％-3％)of H2O2 on MS medium showed the best growth performance,whereas 1％H2O2 on medium containing 1 mg/L 6-BA+1 mg/L NAA exhibited the best promoting effect on seed germination.Using the roots of Senecio tibeticus seedlings as materials for tissue culture,media for callus induction,shoot cluster induction,and rooting induction were prepared,and growth was observed at each stage.Callus was successfully induced,three shoot clusters were differentiated,and each plantlet developed 2-5 roots during rooting induction.The average rooting rate was 70％.Shoot clusters were successfully regenerated from callus derived from the seedling roots of Senecio tibeticus and subsequently rooted.