上海宠物源产ESBLs肺炎克雷伯菌分子特征研究OA

To investigate the resistance phenotypes and molecular characteristics of pet-derived extended-spectrum beta-lactamases(ESBLs)-producing Klebsiella pneumoniae in Shanghai,51 K.pneumoniae isolates from dogs and cats were studied.The micro broth dilution method was used to determine the ESBLs phenotype and the antimicrobial susceptibility of ESBLs-producing K.pneumoniae.Selected strains for whole-genome sequencing to analyse antibiotic resistance genes,virulence factors,ST types,and phylogenetic relationships.The results showed that the isolation rate of ESBLs-producing K.pneumoniae was 35.3%,and these strains were predominantly multidrug resistance(94.4%).The highest resistance rates were observed against Ampicillin(100%),followed by Gentamicin and Ceftiofur(both 94.4%),while the resistance rate to Colistin was the lowest(11.1%).The strains extensively harboured SHV-type β-lactamase resistance genes(100%)and CTX-M-15 genes(40.0%),among others.The virulence factors primarily identified were fimbriae and siderophores,and three high-virulence biomarker genes were detected.ST15 and ST307 were identified as the most prevalent clonal lineages(each accounting for 20%of isolates)and were found to harbour the CTX-M-15,OXA-1,and SHV-28 gene combinations.This study indicates that the antimicrobial resistance situation among ESBLs-producing K.pneumoniae isolated from pets in Shanghai is severe.There is an urgent need to strengthen oversight of veterinary drug use and incorporate pet-derived K.pneumoniae into antimicrobial resistance surveillance systems.