超高效液相色谱-串联质谱法测定鸡蛋中氟苯尼考和氟苯尼考胺残留量的不确定度评价OA

The residues of florfenicol and florfenicol amine in eggs were determined by ultra-performance liquid chromatography-tandem mass spectrometry.By establishing a mathematical model for the uncertainty of the determination of florfenicol and florfenicol amine residues in eggs,the sources of uncertainty were analyzed in sequence,their influence on the uncertainty of the determination results was evaluated,and the combined uncertainty was calculated.Finally,the expanded uncertainty of florfenicol and florfenicol amine residues in eggs was obtained.The results showed that the residue of florfenicol in eggs was 8.03 μg/kg.Under the 95%confidence interval,the combined standard uncertainty was 0.546 and the expanded uncertainty was 1.09(k=2).The final result should be expressed as 8.03±1.09 μg/kg.The residue of florfenicol amine in eggs was 13.2 μg/kg.Under the 95%confidence interval,the combined standard uncertainty was 0.899 and the expanded uncertainty was 1.80(k=2).The final result should be expressed as 13.2±1.80 μg/kg.The evaluation results indicated that the main factors affecting the determination results were the preparation of standard solutions,recovery rate and repeatability measurement.