鹅肥肝形成过程中脂质代谢的甲基化动态调控机制OA

[Objective]The purpose of this study was to reveal the role of DNA methylation regulation in the formation of goose fat liver,in order to further screen and explore the key metabolic pathways and differential methylation genes related to the formation of goose fat liver,so as to improve the epigenetic regulation mechanism of goose fat liver formation.[Methods]In this study,three 70-day-old Lander geese with the same physiological status and health status were selected from the same batch for liver sample collection in the early stage(7 days),middle stage(16 days)and late stage of feeding(25 days).[Results]The results showed that a total of 266.2G of valid data were obtained by sequencing,with an average Q30 of 87.57％,a match rate of about 74％,and a CT conversion efficiency of>99.4％.Among the methylated cytosine detected in the whole genome,the CpG context type accounted for about 65％and the methylation level was about 20％.CHG and CHH types accounted for about 0.7％,and the methylation levels were about 4％and 1.4％,respectively.Principal component analysis showed that the samples at each feeding stage were clearly differentiated.Among the gene function regions,the exon region had the highest methylation level and the lowest promoter region.In the comparison group in the early and middle stage of feeding,524 differential methylated region(DMR)methylation levels increased and 427 DMR methylation levels decreased.In the middle and late stage of feeding,432 DMR methylation levels increased and 236 DMR methylation levels decreased.The differential genes in the early and middle stages were significantly enriched in 121 KEGG pathways,and 829 GO entries were obtained,among which insulin signaling pathway and FoxO signaling pathway were closely related to lipid deposition,involving 6 genes such as MAPK10 and IL6.The differential genes in metaphase and late stage were enriched into 96 KEGG pathways,and 629 GO entries were obtained,among which MAPK signaling pathway and FoxO signaling pathway were significantly enriched,and 13 related genes including MAPK10,PCK1,and PLA2G4A were obtained.[Conclusion]The DMR is mainly enriched in key genes related to lipid metabolism,and its formation is synergisticly regulated by multiple pathways such as FoxO signaling pathway,adipocytokine signaling pathway,PPAR signaling pathway,insulin signaling pathway,etc.,and the methylation levels of key candidate genes MAPK10,PCK1,PLA2G4A are associated with the formation of goose fat liver.