基于气相色谱-质谱法测定大米中2-乙酰基-1-吡咯啉含量的实验项目探索与实践OA

Expanding the experimental teaching project for determining 2-Acetyl-1-pyrroline in Rice by GC-MS in the agri-cultural product quality analysis course.Through the optimization of sample pretreatment methods,mass spectrometry conditions,and methodological comparison and validation,a reliable gas chromatography-mass spectrometry(GC-MS)method for quantifying 2-acetyl-1-pyrroline in rice was successfully established.The results showed that:2-acetyl-1-pyrroline in rice exhibited an ex-cellent linear correlation(R2=0.9999)within the range of 15~1 000 ng/mL.The precision,expressed as relative standard deviation(RSD),ranged from 0.64%to 1.09%.The spike recovery rates were between 87.2%and 98.8%.The method's limit of detection(LOD)and limit of quantitation(LOQ)were determined to be 0.005 μg/g and 0.015 μg/g,respectively,which meets the acceptable criteria for trace analysis.The developed method proved to be operable,sensitive,and precise,making it suitable for the rapid and accurate determination of 2-acetyl-1-pyrroline in rice.Furthermore,this experimental project,with its appropriate level of difficulty,is effective in stimulating students'scientific interest and fostering their innovative thinking and spirit of research explo-ration.