黄芩汤通过PI3K/AKT/NF-κB信号通路缓解溃疡性结肠炎的网络药理学研究OA

Objective To explore the mechanism of Huangqin Decoction in alleviating ulcerative colitis(UC)by regulating the PI3K/AKT/NF-κB signaling pathway based on network pharmacology and animal experiment;To provide basis for its clinical application.Methods Network pharmacology was used to predict the effective components and potential targets of Huangqin Decoction in alleviating UC,and screen core components,targets,and pathways.The binding ability between components and targets was validated through molecular docking.A mouse UC model was constructed using dextran sulfate sodium.The mice were divided into normal group,model group,and Huangqin Decoction group,with 7 mice in each group,and were given corresponding intervention for 7 d.The body mass of mice was monitored,Disease Activity Index(DAI)scoring was performed,colon morphology was observed,and length was measured.HE staining was used to observe the morphology of colon tissue and perform histopathological scoring.ELISA was used to detect the contents of serum pro-inflammatory factors TNF-α,IL-1β and IL-6.Western blot was used to detect the expressions of related pathway proteins in colon tissue.Results Four core components and ten core targets of Huangqin Decoction in alleviating UC were screened,with stable binding verified by molecular docking.Animal experiments showed that Huangqin Decoction could significantly reduce DAI score(P<0.01),restore colon length(P<0.01),alleviate colonic pathological damage(P<0.01),down-regulate serum IL-1β,IL-6 and TNF-α contents(P<0.01),and inhibit p-AKT and p-NF-κB p65 protein expressions in colon tissue(P<0.01,P<0.05).Conclusion Huangqin Decoction can alleviate UC by inhibiting the abnormal activation of PI3K/AKT/NF-κB pathway,reducing inflammatory factor release and intestinal inflammatory injury.