痰热清注射液雾化吸入对慢性支气管炎急性发作大鼠SP、NEP表达影响的实验研究OA

Objective:To investigate the effects of aerosol inhalation of Tanreqing injection on the expression of SP and NEP in rats with acute exacerbation of chronic bronchitis.Methods:60 SD rats were randomly divided into normal control group,model group,Ambroxol group,and low-,medium-and high-dose Tanreqing injection groups,with 10 rats in each group.The rat model of acute exacerbation of chronic bronchitis was established by low-dose lipopolysaccharide(LPS)induction to simulate Gram-negative bacterial infection combined with passive smoking method.The contents of substance P(SP)and neutral endopeptidase(NEP)in serum and bronchoalveolar lavage fluid(BALF)were determined by ELISA.HE staining was used to observe the pathological changes of lung tissue.Immunohistochemistry was applied to detect the protein expressions of SP and NEP in lung tissue,followed by semi-quantitative scoring.Results:Compared with the model group,the levels of SP were significantly decreased,while the levels of NEP were significantly increased in serum and BALF of the ambroxol group and all Tanreqing injection treatment groups(P<0.05).Immunohistochemical results showed that,compared with the model group,the protein expression of SP was downregulated and NEP was upregulated in the lung tissues of each treatment group,with statistically significant differences(P<0.05).In addition,the high-dose Tanreqing Injection group exhibited significant differences in SP and NEP protein expression compared with the ambroxol group(P<0.05).Conclusion:Aerosol inhalation of Tanreqing Injection can effectively reduce the levels of SP in serum and BALF,as well as the protein expression of SP in lung tissue,while increase the contents of NEP in serum,BALF and lung tissue.This regulatory effect helps alleriate airway neurogenic inflammation.