龙砂医派黄连汁炒酸枣仁的HPLC指纹图谱和多成分含量测定方法研究OA
Study on HPLC fingerprint and multi-component determination of Ziziphus jujuba stir-fried with Coptis chinensis juice from Longsha Medical School
目的 建立龙砂医派特色炮制品种黄连汁炒酸枣仁的高效液相色谱(HPLC)指纹图谱及多成分含量测定方法,为该特色品种的传承与临床应用提供依据.方法 采用HPLC法建立12批黄连汁炒酸枣仁样品的指纹图谱,并进行相似度评价和共有峰指认,然后对样品中的乌药碱、木兰花碱、盐酸黄连碱、表小檗碱、盐酸药根碱、斯皮诺素、盐酸小檗碱、盐酸巴马汀、6"'-阿魏酰斯皮诺素、酸枣仁皂苷A进行含量测定.结果 12批样品指纹图谱的相似度均大于0.960;共标定出17个共有峰,指认了其中9个共有峰,分别为乌药碱、木兰花碱、盐酸黄连碱、表小檗碱、盐酸药根碱、斯皮诺素、盐酸小檗碱、盐酸巴马汀、6"'-阿魏酰斯皮诺素.含量测定结果显示,12批样品中乌药碱、木兰花碱、盐酸黄连碱、表小檗碱、盐酸药根碱、斯皮诺素、盐酸小檗碱、盐酸巴马汀、6"'-阿魏酰斯皮诺素、酸枣仁皂苷A的质量分数分别为0.044～0.091、0.908～1.698、0.325～0.549、0.163～0.287、0.103～0.172、0.712～0.952、1.185～1.843、0.341～0.598、0.228～0.405、0.050～0.091 mg/g.结论 建立的HPLC指纹图谱及多成分含量测定方法稳定、可靠,可为黄连汁炒酸枣仁的质量标准制定及临床应用提供依据.
OBJECTIVE To establish a high-performance liquid chromatography(HPLC)fingerprint and a multi-component content determination method for Ziziphus jujuba stir-fried with Coptis chinensis juice,a characteristic processed product of Longsha Medical School,and to provide a basis for the inheritance and clinical application of this distinctive variety.METHODS HPLC method was employed to establish the fingerprints of 12 batches of Z.jujuba stir-fried with C.chinensis juice.Similarity evaluation and common peak identification were subsequently carried out.Quantitative determination was performed on coclaurine,magnoflorine,coptisine hydrochloride,epiberberine,jatrorrhizine hydrochloride,spinosin,berberine hydrochloride,palmatine hydrochloride,6 "'-feruloylspinosin,and jujuboside A of samples.RESULTS The fingerprint similarities of all 12 batches of samples were higher than 0.960.A total of 17 common peaks were calibrated,among which 9 peaks were identified as coclaurine,magnoflorine,coptisine hydrochloride,epiberberine,jatrorrhizine hydrochloride,spinosin,berberine hydrochloride,palmatine hydrochloride,and 6 "'-feruloylspinosin,respectively.The quantitative results showed that the mass fractions of coclaurine,magnoflorine,coptisine hydrochloride,epiberberine,jatrorrhizine hydrochloride,spinosin,berberine hydrochloride,palmatine hydrochloride,6 "'-feruloylspinosin,and jujuboside A in the 12 batches of samples were 0.044-0.091,0.908-1.698,0.325-0.549,0.163-0.287,0.103-0.172,0.712-0.952,1.185-1.843,0.341-0.598,0.228-0.405,and 0.050-0.091 mg/g,respectively.CONCLUSIONS The established HPLC fingerprint and multi-component determination method is stable and reliable,and can provide a basis for the quality standard formulation and clinical application of Z.jujuba stir-fried with C.chinensis juice.
秦苹;张逸婷;卞振华;陆颖颖;张文明;冯子芳;顾丽红;夏晨洁;胡敏敏;陈晓伟;陆兔林
无锡市中医医院药学部,江苏 无锡 214071||江苏省中医流派研究院龙砂医派分院,江苏 无锡 214071无锡市中医医院药学部,江苏 无锡 214071无锡市中医医院药学部,江苏 无锡 214071无锡市中医医院药学部,江苏 无锡 214071无锡市中医医院药学部,江苏 无锡 214071无锡市中医医院药学部,江苏 无锡 214071无锡市中医医院药学部,江苏 无锡 214071无锡市中医医院药学部,江苏 无锡 214071无锡市中医医院药学部,江苏 无锡 214071无锡市中医医院药学部,江苏 无锡 214071南京中医药大学药学院,南京 210023
医药卫生
黄连汁炒酸枣仁龙砂医派指纹图谱高效液相色谱法以药制药含量测定质量控制
Ziziphus jujuba stir-fried with Coptis chinensis juiceLongsha Medical Schoolfingerprint chromatogramHPLC methodprocessing medicinals with medicinalscontent determinationquality control
《中国药房》 2026 (15)
1974-1978,5
国家重点研发计划项目(No.2023YFC3504200)江苏省中医流派研究院开放课题(No.JSZYLP2024033,No.JSZYLP2024028)江苏省中医药科技发展计划项目(No.MS2023066)江苏省中药骨干人才高级研修项目(No.苏中医科教[2025]11号)江苏省药学会-奥赛康医院药学科研项目(No.A202525)南京中医药大学自然科学基金项目(No.XZR2024325)
评论