基于标准煎液的红芪金颗粒提取工艺研究OA

OBJECTIVE To study the extraction process of Hongqijin granules with the standard decoction of Hongqijin formula as the reference.METHODS Fifteen batches of Hongqijin formula standard decoction were prepared.The reference characteristic chromatogram was established,and the chromatographic similarity was calculated.The average extraction yield and average transfer rates of calycosin-7-glucoside,salidroside,curcumin,and emodin of the 15 batches of standard decoction were determined.Taking soaking time,water addition amount,and decoction time as influencing factors,and extraction yield,transfer rates of marker components,as well as chromatographic similarity with the reference characteristic chromatogram as evaluation indexes,orthogonal test combined with principal component analysis was adopted to optimize the extraction process of Hongqijin granules,followed by process verification.RESULTS The average extraction yield of 15 batches of Hongqijin formula standard decoction was 32.55%.The average transfer rates of calycosin-7-glucoside,salidroside,curcumin,and emodin were 70.68%,74.35%,2.16%,and 4.62%,respectively.The optimal extraction process of Hongqijin granules was confirmed as follows:extraction was carried out twice;the first extraction used 8-fold water for 60 min,and the second extraction adopted 6.5-fold water for 45 min.In three validation tests,the average extraction yield was 33.01%.The average transfer rates of the four marker components were 79.51%,75.54%,1.84%,and 4.30%,with all RSD values less than 2%.The chromatographic similarities between the extracting solution and reference characteristic chromatogram were all higher than 0.990.CONCLUSIONS The optimized extraction process of Hongqijin granules exhibits good stability,and the material basis of the extracting solution is consistent with that of Hongqijin formula standard decoction.