哈它各其-7联合胡麻油通过调控炎症反应与GSK3β信号通路促进压疮愈合的研究OA

Objective This study aimed to explore the potential mechanisms of the Mongolian medicine hatagaqi-7 combined with sesame oil in promoting pressure ulcer wound healing based on network pharmacology,and to verify the findings through molecular docking and animal experiments.Methods Potential active components and targets of hatagaqi-7 combined with sesame oil,as well as pressure ulcer-related targets,were identified through database and literature searches.The common targets were used to construct a protein-protein interaction(PPI)network,followed by gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)pathway analyses,and molecular docking.A rat model of pressure ulcer was established and divided into six groups:control(K),model(M),hatagaqi-7(Q),sesame oil(H),hatagaqi-7 combination sesame oil(H+Q)and yunnan baiyao(Y)groups(n=10).All groups received corresponding treatments for 14 d.Wound morphology,healing rate,histopathological changes,and the expression of interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),glycogen synthase kinase-3β(GSK3β),NF-κB p65 and β-catenin were evaluated.Results Network pharmacology identified 35 active compounds,644 drug targets,and 398 disease targets.Core targets included BCL2,TNF-α,NFKB1,IL-6,IL-1β,and GSK3β,mainly involving the TNF inflammatory response,and Wnt signaling pathways.Molecular docking indicated that the major active components-caffeine and estradiol-exhibited strong binding affinities with GSK3β,TNF-α,IL-1β,and IL-6.Animal experiments showed that the(H+Q)group had the highest wound healing rate and the most abundant collagen deposition.The expression levels of IL-1β,IL-6,TNF-α,GSK3β,and NF-κB p65 were significantly downregulated in the(H+Q)group(P＜0.05),with greater reductions compared with the H,Q,Y groups(P＜0.05).The expression of β-catenin was significantly upregulated in the(H+Q)group(P＜0.05),and the magnitude of upregulation was significantly greater than that in the H,Q,Y groups(P＜0.05).Conclusions Hatagaqi-7 combined with sesame oil significantly promotes pressure ulcer healing,potentially through suppressing inflammatory responses and regulating the GSK3β signaling pathway.The combination therapy demonstrates potential clinical value and provides an experimental basis for the application of mongolian medicine in pressure ulcer treatment.