超高效液相色谱-串联质谱法同时测定人全血中10种精神类药物浓度研究OA

Objective To establish a rapid and accurate ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)method for the determination of 10 common abused drugs and antipsychotics including meth-amphetamine,ketamine and clozapine in human whole blood.Methods Blood samples were pretreated using an acetonitrile protein precipitation method.Isocratic elution was carried out using the mobile phase system consisting of 0.1％formic acid containing 20 mmol/L ammonium acetate aqueous solution and acetonitrile.The injection volume was 1 μL with a flow rate of 0.2 mL/min.An electrospray ionization source(ESI)in positive ion mode and multiple reaction monitoring(MRM)scan-ning were applied,and internal standard quantification was used.The total analysis time was 4 min.Meanwhile,the specific-ity,linear range,limit of detection,accuracy and precision,extraction recovery and carryover contamination of the method were validated.Results The 10 drugs showed correlation coefficients(r)>0.995 within their respective concentration ran-ges.The method demonstrated limits of detection(LOD)of 2.0-3.5 ng/mL and lower limits of quantification(LLOQ)at 10.00 ng/mL.Accuracy ranged from 98.7％to 110.6％at low,medium,and high spiked concentrations,with both intra-day and inter-day precision(expressed as RSD)below 10.00％.Extraction recoveries were between 83.35％and 110.95％.Conclusion This method demonstrates simplicity,rapidity,good reproducibility,low detection limits,and strong specifici-ty.It is suitable for rapid quantitative analysis of common abused drugs and antipsychotics.