基于巨噬细胞极化及p38 MAPK信号通路探讨黄芪建中汤合小承气汤治疗术后肠梗阻的机制OA

Objective It is to investigate the therapeutic mechanism of Huangqi Jianzhong Decoction combined with Xi-aochengqi Decoction in the treatment ofpostoperative ileus(POI).Methods Twenty-four SPF-grade male Wistar rats were randomly divided into blank control group,model group,herbal enema group and soap-water enema group,with 6 rats in each group.The rats of all groups except for the blank group were prepared to establish models of POI.In 24 hours after surgery,the herbal enema group was given Huangqi Jianzhong Decoction combined with Xiaochengqi Decoction via enema,the soap-water enema group was given soap-water via enema,all once daily,continuously treated fo 7 days.The intestinal transit rate of rats in each group was detected,their Butzner scores of intestines were assessed,the levels of interleukin(IL)-6,tumor necrosis factor-α(TNF-α),IL-10,CD86,and CD206 in abdominal aortic blood were measured by ELISA,and the protein expressions of p38 mitogen-activated protein kinase(p38 MAPK)and phosphorylated p38 MAPK(p-p38 MAPK)in small intestinal tissue were detected by Western blot method.Results Compared with the control group,the intestinal transit rate of rats in the model group was significantly reduced(P<0.05),while the Butzner scores and ser-um levels of IL-6,TNF-α,IL-10,CD86 and CD206,as well as the relative protein expressions of p38 MAPK and p-p38 MAPK in small intestinal tissue were significantly increased(all P<0.05).There was no significant difference in intesti-nal transit rate of rats among the model group,herbal enema group and soap-water enema group(all P>0.05),the Butzner score,serum levels of IL-6,TNF-α,CD86 and CD206,and the relative protein expressions of p38 MAPK and p-p38 MAPK in small intestinal tissue were all significantly lower(all P<0.05),while the serum level of IL-10 was signifi-cantly higher in the herbal enema group than those in the model group(P<0.05);the Butzner score,serum levels of IL-6,TNF-α and CD206,and the relative protein expressions of p38 MAPK and p-p38 MAPK in small intestinal tissue were all significantly lower in the soapy-water enema group than those in the model group(all P<0.05).Furthermore,the Butzner scores and the relative protein expressions of p38 MAPK and p-p38 MAPK in small intestinal tissue were signifi-cantly lower,while the serum levels of IL-10 and CD206 were significantly higher in the herbal enema group than those in the soap-water enema group(all P<0.05).Conclusion Huangqi Jianzhong Decoction combined with Xiaochengqi Decoc-tion can effectively alleviate intestinal inflammation and promote gastrointestinal functional recovery in POI rats via regula-ting macrophage polarization and the p38 MAPK signaling pathway.