清肝健脾活血方对CCl4诱导肝纤维化大鼠的保护作用研究OA

Objective It is to observe the hepatoprotective effect of prescription for clearing liver,fortifying spleen and resolving stasis(PCLFSRS)on hepatocytes in rats with liver fibrosis induced by CCl4.Methods A total of 72 male SD rats were randomly divided into normal group,model group,high-dose,medium-dose and low-dose groups of PCLFSRS,Biejia-jian Pill(BJJP)group,with 6 rats in each group.The rats of all the groups except for the normal group were prepared with intraperitoneal injection of 40%CCl4 olive oil suspension to establish models of liver fibrosis.Starting from the 2rd day of modeling,high-dose,medium-dose and low-dose groups of PCLFSRS were administered PCLFSRS 32.084 g/kg,16.042 g/kg,and 8.021 g/kg via gavage,respectively,while the BJJP group was administered BJJP 0.9255 g/kg via gavage,the normal group and model group were given pure water via gavage,all once daily,continuously treated for 8 weeks.The pathological morphology of liver tissue was observed by HE staining,Masson staining,and Sirius red staining.The serum levels of ala-nine aminotransferase(ALT),aspartate aminotransferase(AST),albumin(ALB),lactate dehydrogenase(LDH),total bilirubin(TBil),hyaluronic acid(HA),laminin(LN),type Ⅲ procollagen(PC-Ⅲ),and type IV collagen(Ⅳ-C),as well as the contents of hydroxyproline(Hyp),glutathione peroxidase(GPX),and glutathione S-transferase(GST)in liver tissue were measured by the kit methods,the apoptosis of hepatocytes was assessed by TUNEL method,and the protein ex-pressions of Bax and Caspase-3 in liver tissue were detected by Western blot.Results Compared with the normal group,in-flammatory cell aggregation with fibrous septa formation in liver tissue were found in the rats of the model group,the serum levels of AST/ALT,LDH,TBil,HA,LN,PC-Ⅲ and Ⅳ-C,as well as then contents of Hyp and GST in liver tissue were significantly increased(all P<0.05),while the serum level of ALB and content of GPX in liver tissue were significantly decreased(all P<0.05),the apoptosis rate and relative protein expressions of Bax and Caspase-3 in liver tissue were sig-nificantly increased(all P<0.05).Compared with the model group,the fibrosis of liver tissue in the rats of the hight-dose group of PCLFSRS and BJJP group were obviously alleviated,the serum levels of AST/ALT,LDH,TBil,HA,LN,PC-Ⅲand Ⅳ-C,as well as then contents of Hyp and GST in liver tissue were significantly decreased(all P<0.05),while the contents of GPX in liver tissue were significantly decreased(all P<0.05),the apoptosis rates and relative protein expres-sions of Bax and Caspase-3 in liver tissue were significantly decreased(all P<0.05),the serum level of ALB was signifi-cantly increased,while the relative protein expression of Bax in liver tissue was significantly decreased in the high-dose group of PCLFSRS.Conclusion PCLFSRS can significantly alleviate CCl4 induced liver injury in rats,reduce liver cell ap-optosis,and protect liver cells.