中药紫苏根质量标准的研究OA

Objective To establish a quality standard for slices of Radix Perillae and provide a scientific basis for its standardized appli-cation.Methods Fifteen batches of Radix Perillae slices from different sources were studied.Using rosmarinic acid as the indicator com-ponent,qualitative identification was conducted by thin-layer chromatography(TLC),and its content was quantified by high-performance liquid chromatography(HPLC).Routine tests including moisture,total ash,acid-insoluble ash,and extractives were per-formed according to the methods specified in Chinese Pharmacopoeia(中国药典).Results TLC analysis showed that 13 batches(except ZSG0001 and ZSG0009)exhibited clear blue fluorescent spots corresponding to the rosmarinic acid reference,with good resolu-tion.The results of the routine tests were:moisture,7.4%-10.4%(proposed limit:≤11.0%);total ash,2.0%-4.6%(proposed limit:≤5.0%);acid-insoluble ash,below 0.50%in all samples(no limit specified);extractives,6.0%-9.0%(proposed limit:≥5.0%).All batches complied with the proposed criteria for these items.The content of rosmarinic acid determined by HPLC ranged from 0.044%to 0.384%,with a mean value of 0.135%.Based on these results,a content limit of not less than(≥)0.05%was pro-posed,which was met by 13 batches(excluding ZSG0001 and ZSG0009).Conclusion A comprehensive quality evaluation system encompassing physicochemical tests,TLC identification,and content determination was established for Radix Perillae slices through analysis of 15 batches from different sources.This system complements existing local standards by providing more comprehensive indica-tors,thereby enabling effective quality control,ensuring clinical safety and efficacy,and offering technical support for the standardized cultivation,processing,and medicinal use of this herb.