基于Wnt/β-catenin信号通路探讨搜风愈喘方改善哮喘大鼠气道重塑的作用机制OA

Objective To explore the effect of the Soufeng Yuchuan Formula(搜风愈喘方,SFYCF)on airway remodeling in asthma model rats and clarify its mechanism of mediating epithelial-mesenchymal transition(EMT)by regulating the Wnt/β-catenin signaling pathway.Methods Forty-eight 6-8-week-old specific pathogen free(SPF)rats were randomly divided into the blank group,the model group,the dexamethasone group,the low/medium/high-dose SFYCF groups,with 8 rats in each group.After establishing an asthma model with aluminum hydroxide adjuvant,the rats were administered with corresponding drugs for 3 weeks before sampling.The general conditions of the rats were observed,and HE and Masson staining were used to observe lung tissue pathology.The levels of IFN-γ,IL-4,IL-1β,and MMP-9 in sera were detected by ELISA.RT-PCR was used to detect the mRNA expressions of Wnt5a,Wnt7a,β-catenin,GSK-3β,C-Myc,and E-cadherin in the lung tissue,while Western blot(WB)was used to detect the protein expressions of Wnt5a,β-catenin,GSK-3β,and Vimentin in the lung tissue.Results Compared with the model group,the body weight of rats in the SFYCF groups was increased(P<0.05,P<0.01),and their symptom scores such as nose scratching and wheezing were improved(P<0.01);airway inflammation scores and collagen deposition rates were decreased(P<0.01);serum IFN-γ levels were increased(P<0.05,P<0.01),while IL-4,IL-1β,and MMP-9 levels were decreased(P<0.01);mRNA expressions of Wnt5a,Wnt7a,β-catenin,GSK-3β,and C-Myc in lung tissue were decreased(P<0.05,P<0.01),while E-cadherin mRNA expression was increased(P<0.05,P<0.01);protein expressions of Wnt5a,β-catenin,GSK-3β,and Vimentin were decreased(P<0.01).Conclusion SFYCF may affect EMT by regulating the Wnt/β-catenin signaling pathway,thereby ameliorating airway remodeling and exerting a therapeutic effect on asthma.