circFBLIM1通过调控糖酵解miR-338/PFKL轴促进肝癌细胞增殖并抑制凋亡OA

Objective To investigate the regulatory effect of circular RNA circFBLIM1 on the proliferation,apoptosis and migration of liver cancer cells,and elucidate the molecular mechanism by which it affects liver cancer progression via miR-338/phosphofructoki-nase liver type(PFKL)-mediated glycolysis,so as to provide a novel theoretical basis for targeted metabolic intervention of liver cancer.Methods Quantitative polymerase chain reaction(qPCR)was used to detect the expression of circFBLIM1 in two human liver cancer cell lines(SNU-387 and Huh-7)and a human normal hepatocyte line(THLE-2).siRNA was applied to knock down circFBLIM1 and pcDNA vector was used to overexpress circFBLIM1 in SNU-387 cells.Flow cytometry,Cell Counting Kit-8(CCK-8),and wound healing assay were performed to analyze cell apoptosis,proliferation and migration.qPCR and Western blot were adopted to detect the influences of circFBLIM1 knockdown and overexpression on the expressions of miR-338 and PFKL in SNU-387 cells.Results circFBLIM1 was highly expressed in SNU-387 and Huh-7 cells(both P＜0.01).Silencing circFBLIM1 in SNU-387 cells significantly inhibited cell prolif-eration,promoted apoptosis,reduced migration ability,up-regulated miR-338 expression,and down-regulated PFKL mRNA and protein expressions(all P＜0.05).Overexpressing circFBLIM1 promoted cell proliferation,enhanced migration ability,down-regulated miR-338,and up-regulated PFKL mRNA and protein expressions(all P＜0.05).Conclusions circFBLIM1 is highly expressed in liver cancer cells.circFBLIM1 facilitates the proliferation and migration of liver cancer cells and exerts its effects in liver cancer progression by activating the circFBLIM1/miR-338/PFKL axis in the glycolytic pathway.