不同水平的黑水虻替代豆粕对育成期五黑鸡的生长性能、血液指标及肠道健康的影响OA

This study aimed to investigate the effects of substituting soybean meal(SBM)with dif-ferent levels of black soldier fly(BSF)larvae meal in the diet on the growth performance,blood parameters,and intestinal health of Wuhei chick-ens during the rearing period.A total of 300 healthy Wuhei chickens aged 70 days with an average body weight of(0.97±0.11)kg were ran-domly assigned to five treatment groups,each with six replicates.The control group(CON,BSF0)received a corn-soybean meal basal diet,while the experi-mental groups were fed diets in which 25%(BSF25),50%(BSF50),75%(BSF75),or 100%(BSF100)of the SBM was replaced by BSF meal.The trial lasted 35 d,including a 7 d adaptation period and a 28 d experi-mental period.The results showed:① compared with the CON group,the BSF25 group significantly increased the average daily gain of Wuhei chickens during the rearing period from days 15 to 28(P<0.05).The BSF50 and BSF75 groups showed extremely significantly increased albumin levels(P<0.01).During days 0-14 of the trial,the BSF50 group exhibited significantly decreased globulin levels(P<0.05).② Com-pared with the CON group,the BSF75 group extremely significantly increased the spleen index of Wuhei chickens during the rearing period(P<0.01).During days 0-14,serum IgG and IgA levels in the experimen-tal groups were extremely significantly increased(P<0.01),and serum IgM levels in the BSF50,BSF75,and BSF100 groups were significantly increased(P<0.05).During days 15-28,serum IgG levels in the experi-mental groups were extremely significantly increased(P<0.01),serum IgM levels in the BSF50 and BSF75 groups were extremely significantly increased(P<0.01),and serum IgA levels in the BSF50,BSF75,and BSF100 groups were significantly increased(P<0.05).③ Compared with the CON group,during days 0-14,the BSF50 group showed significantly decreased serum malondialdehyde levels(P<0.05),while total antioxi-dant capacity and glutathione peroxidase activity in the experimental groups were extremely significantly increased(P<0.01).During days 15-28,serum glutathione peroxidase and catalase activities in the experi-mental groups were extremely significantly increased(P<0.01);serum total antioxidant capacity in the BSF75 group was significantly increased(P<0.05);and serum superoxide dismutase activity in the BSF50,BSF75,and BSF100 groups was extremely significantly increased(P<0.01).④ Compared with the CON group,during days 0-14,serum endothelin levels in the BSF50 group were significantly decreased(P<0.05),and serum diamine oxidase levels in the BSF50,BSF75,and BSF100 groups were significantly decreased(P<0.05).During days 15-28,serum diamine oxidase levels in the BSF75 group were extremely significantly lower than those in the CON group(P<0.01).The crypt depth in the BSF25 and BSF100 groups was extremely significantly increased(P<0.01),and the intestinal wall thickness in the BSF25 group was extremely significantly increased(P<0.01).Substituting 25%of the soybean meal in the basal diet with black soldier fly(Hermetia illucens)larvae meal is considered optimal for promoting growth and improving production performance in Wuhei chickens during the grower phase.