基于PI3K/Akt/NF-κB通路探讨羌活膏摩法对膝骨关节炎大鼠作用机制OA

Objective:To explore the mechanism of Qianghuo ointment Tuina in improving knee osteoarthritis(KOA).Methods:The KOA model was established in SD rats by intra-articular injection of papain.Lequesne MG score was used to evaluate knee joint function after model establishment and treatment completion.HE staining of knee synovial tissue was performed to observe morphological changes of cartilage and synovium.The serum levels of IL-1β,TNF-α and IL-10 were detected by ELISA.Expression of PI3K-Akt pathway-related proteins(p-PI3K/PI3K,p-Akt/Akt,p-NF-KB/NF-KB)in knee synovial tissue was measured by Western blot.mRNA expression levels of IL-4,IL-10 and COMP in synovium were determined by real-time fluorescent quantitative PCR.Results:Behavioral results showed that compared with the model group,Lequesne MG score was significantly decreased in the treatment group(P＜0.001),with no statistically significant difference compared with the blank group(P＞0.05).HE staining re-vealed alleviated cartilage damage and reduced synovial inflammatory infiltration in rats after intervention with Qian-ghuo ointment Tuina.ELISA results demonstrated that compared with the model group,the serum contents of pro-inflammatory factors IL-1β and TNF-α were notably decreased,while the content of anti-inflammatory factor IL-10 was remarkably increased in the treatment group(P＜0.001).Western blot and RT-qPCR results indicated that pro-tein expression levels of p-PI3K,p-Akt and NF-κB in knee synovial tissue were significantly down-regulated(P＜0.05),mRNA levels of IL-4 and IL-10 were elevated,and mRNA expression of COMP was markedly reduced in the treatment group relative to the model group(P＜0.05).Conclusion:Qianghuo ointment Tuina can effectively attenu-ate inflammation and improve joint function in KOA rat models.Its mechanism may be related to the inhibition of PI3K/Akt/NF-κB signaling pathway activation.